30,808 results

1. pOGG216

   Plasmid

   #114002

   Purpose

   Level 2 golden gate cloning vector for medium copy number free-living expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRiboI-APEX2m

   Plasmid

   #176843

   Purpose

   Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Use

   Integrating

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTTH+H1.4ΔCTD

   Plasmid

   #157797

   Purpose

   Expression of human linker histone H1.4 without c-terminal tail

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4ΔCTD (H1-4 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   pTAC

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b-eGO-Matryoshka-LE-LP

   Plasmid

   #100017

   Purpose

   Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpEGFP-LSSmOrange
   

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSA120

   Plasmid

   #59789

   Purpose

   C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   hsp-16.41 promoter
   zif-1 coding
   gpd-2/gpd-3 intergenic sequence
   mCherry (worm)
   unc-54 3' UTR
   

   Expression

   Bacterial

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP974-1

   Plasmid

   #99497

   Purpose

   TEV::linker::tagRFP::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::linker::tagRFP::3XFlag
   

   Expression

   Bacterial

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET:GACT:GFP

   Plasmid

   #60734

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-ybbR-HIS-sfGFP-DocI

   Plasmid

   #58708

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   sfGFP-DocI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLN

   Plasmid

   #40028

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   loxP
   Neo
   

   Expression

   Bacterial and Mammalian

   Promoter

   None and SV40

   Available Since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT-β3

    Plasmid

    #166579

    Purpose

    For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

    Depositor

    Vesa Hytönen

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

    Insert

    β3 (Itgb3 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD3-proD/mClover/nat

    Plasmid

    #120330

    Purpose

    Bacterial mClover labeling

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mClover
    

    Tags

    D103N (see comment below)

    Expression

    Bacterial

    Promoter

    (None)

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC-MBP-TEV-GG

    Plasmid

    #215794

    Purpose

    Cloning of target gene with N terminal MBP tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHflu2

    Plasmid

    #203880

    Purpose

    pHI1 with insertion of ampicillin-resistance gene

    Depositor

    Bogumil Karas

    Insert

    broad-spectrum class A beta-lactamase TEM-116
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE160

    Plasmid

    #118508

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Expression

    Bacterial

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTDpelB-CTwinStrep

    Plasmid

    #45941

    Purpose

    use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    PelB and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gma-pCAMBIA-1301-STTM171

    Plasmid

    #84080

    Purpose

    Disturb the expression of miR171 in soybean

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR171
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+) 6xHis-TEV-IFIT2

    Plasmid

    #53558

    Purpose

    bacterial expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. GST-Cav FL (1-178)

    Plasmid

    #22445

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

    Insert

    Caveolin1(1-178)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. ACOT11A

    Plasmid

    #42424

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACOT11A (ACOT11 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pXY1-spc

    Plasmid

    #90488

    Purpose

    Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

    Depositor

    Sheng Yang

    Article

    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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