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pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Publication

van der Linden et al eLife. 2025 Dec 19;14:RP105086. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

jRCaMP1b

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-His-zBasic-RBD

Plasmid

#194999

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli

Depositor

Marko Hyvönen

Publication

Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

Insert

RBD (S SARS-CoV-2)

Tags

His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Publication

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Publication

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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enz.083-NMT1

Plasmid

#154047

Purpose

Expresses NMT1 in E. coli

Depositor

Christopher Voigt

Publication

Wallace et al Advanced Functional Materials 2020

Insert

NMT1 (N-myristoyltransferase 1) (NMT1 Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pXD69m1-TetW

Plasmid

#191246

Purpose

E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD69m2-AphA

Plasmid

#191247

Purpose

E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

Plasmid

#191650

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

Plasmid

#192273

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mTagBFP2

Plasmid

#191746

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Publication

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mTagBFP2

Expression

Bacterial

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET29-hMIGA2(170-593)-6xhis

Plasmid

#192834

Purpose

Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Publication

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

6xhis

Expression

Bacterial

Promoter

T7

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJLG029

Plasmid

#192980

Purpose

BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Publication

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

pruR-3xFLAG

Tags

3XFLAG

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE075

Plasmid

#176832

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

714

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAM2314-RbcS-mNG

Plasmid

#165582

Purpose

Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

Depositor

Daniel Ducat

Publication

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen

Expression

Bacterial

Promoter

Prbcs

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-131

Plasmid

#185923

Purpose

Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

Depositor

Mitja Remus-Emsermann

Publication

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mTurquoise2

Expression

Bacterial

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Publication

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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