31,828 results
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Plasmid#41685DepositorInsertPhoRadA intein
TagsGB1 and His-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #1
Plasmid#104208PurposeBacterial expression of WW domain #1 from NEDD4DepositorInsertNEDD4 WW domain #1 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
TagsTwinStrep, 10xHisExpressionBacterialPromoterT7Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetR193-Wasabi
Plasmid#86105PurposemWasabi reporter gene gated by temperature-sensitive TetR I193NDepositorInsertsTetRts
mWasabi
Tags6xHisExpressionBacterialMutationI193NPromoterptetA and ptetRAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLitR-L1
Plasmid#74683PurposeLitR-L1 gateDepositorInsertPTac-RBSL1-LitR-PLitR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2x-MT3
Plasmid#32115DepositorAvailable SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MAD2L1_HORMA
Plasmid#178480PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.0
Plasmid#81050Purposebacterial expression of murine TDGDepositorAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK1_eGFP
Plasmid#59853PurposeConstitutively expresses eGFP with proK1 promoterDepositorInsertEnhanced Green Fluorescent Protein
Tags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK1Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAJM.721
Plasmid#108522PurposePSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.DepositorInsertPSalTTC-YFP
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196MAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationD193EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
Tags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA13c-RFP
Plasmid#126098PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23102Available SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c006
Plasmid#139755PurposeFor protein expression/crystallography (PDB:6F59)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
TagsHis6ExpressionBacterialMutationG177D; contains only amino acids E41-S224PromoterT7Available SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only