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32,457 results

Showing: 10201 - 10220 of 32457 results

  2. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

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  7. TAF1

    Plasmid
    #39118
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. GLRX5

    Plasmid
    #39007
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX5 (GLRX5 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  14. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  17. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  18. Clover2-pBAD

    Plasmid
    #54576
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and Clover2
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pTXB1-CII

    Plasmid
    #51566
    Purpose
    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cII
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51566-plasmid-map-sequence-id-78825

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Showing: 10201 - 10220 of 32457 results