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34,239 results

Showing: 10221 - 10240 of 34239 results

  1. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184738
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2
    Depositor
    Randall Platt
    Publication
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184738-plasmid-map-sequence-id-361531

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  2. pGJJ055

    Plasmid
    #183622
    Purpose
    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.
    Depositor
    Ben Lehner
    Publication
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183622-plasmid-map-sequence-id-361477

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  4. pHB-2

    Plasmid
    #140955
    Purpose
    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.
    Depositor
    Kang Zhou
    Publication
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Expression
    Bacterial
    Mutation
    R238S mutation in PhaC
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140955-plasmid-map-sequence-id-334168

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  6. pFTK093

    Plasmid
    #171365
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171365-plasmid-map-sequence-id-352204

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  10. KTK_354

    Plasmid
    #180525
    Purpose
    KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.
    Depositor
    Tom Ellis
    Publication
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180525-plasmid-map-sequence-id-358850

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  12. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid
    #181789
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181789-plasmid-map-sequence-id-354816

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  20. pSL1-E2C

    Plasmid
    #180421
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.
    Depositor
    Nancy Moran
    Publication
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    E2 Crimson
    Expression
    Bacterial
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180421-plasmid-map-sequence-id-357219

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Showing: 10221 - 10240 of 34239 results