30,808 results

1. pET28-sfGFP-DogTag Loop B

   Plasmid

   #171780

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop B
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Asp1…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL

   Plasmid

   #119952

   Purpose

   Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   gpps
   limS
   

   Expression

   Bacterial

   Mutation

   N-terminal truncation of 56 amino acid residues (…

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. attB Neo* attP

   Plasmid

   #138412

   Purpose

   attB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Mouse Targeting; Att/phic31

   Expression

   Bacterial and Mammalian

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-At_PRORP2-His

   Plasmid

   #67870

   Purpose

   bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP2 (PRORP2 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_Nsp7

   Plasmid

   #156476

   Purpose

   Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD280.7

   Plasmid

   #173172

   Purpose

   Expresses SO_4387

   Depositor

   Jeffrey Tabor

   Article

   Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   SO_4387
   

   Expression

   Bacterial

   Promoter

   J23107

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MSP1D1deltaH4/2H5

   Plasmid

   #71715

   Purpose

   Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-RP2

   Plasmid

   #86072

   Purpose

   A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   RP2 (RP2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-T5-FB-E25TAG

   Plasmid

   #176545

   Purpose

   Expresses IgG affinity protein FB with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change glutamic acid 25 to amber stop codon

   Promoter

   T5

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE60 Trx1 CSAAA

    Plasmid

    #98401

    Purpose

    Bacterial expression vector for Trx1 CSAAA

    Depositor

    Tobias Dick

    Article

    Schwertassek et al EMBO J. 2007 Jul 11;26(13):3086-97. Epub

    Insert

    Trx1 CSAAA (TXN Human)
    

    Expression

    Bacterial

    Mutation

    C35S, C62A, C69A, C73A

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXDC61-RalF

    Plasmid

    #21844

    Depositor

    Howard Shuman

    Article

    Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

    Insert

    RalF
    

    Tags

    TEM

    Expression

    Bacterial

    Available Since

    Sept. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. B7-3

    Plasmid

    #111491

    Purpose

    Tyr-tRNA synthetase

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Tyr-tRNA synthetase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTara:500*

    Plasmid

    #107954

    Purpose

    Arabinose-inducible T7*RNAP

    Depositor

    Jim Haseloff

    Article

    Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

    Insert

    T7RNAP (modified)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    R632S

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVHed05

    Plasmid

    #82351

    Purpose

    Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR

    Depositor

    Richard Murray

    Article

    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

    Inserts

    TP901-1 integrase
    Bxb1 integrase
    AraC (araC )
    TetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFPT7

    Plasmid

    #28012

    Depositor

    Lingchong You

    Article

    Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

    Insert

    T7 RNAP
    

    Expression

    Bacterial

    Available Since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP2774

    Plasmid

    #160667

    Purpose

    Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    gmc6 (gfp) sgRNA
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-His6-Sumo-IntNtr-eSrt2A

    Plasmid

    #126015

    Purpose

    Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-IntNtr-eSrt2A
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSS2 (hS100B)

    Plasmid

    #26774

    Depositor

    Gary Shaw

    Article

    Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14

    Insert

    S100B (S100B Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRC1765

    Plasmid

    #141346

    Purpose

    Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

    Depositor

    Rafael Fernandez-Leiro

    Article

    Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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