30,807 results

1. GST-PRDM16 1039-1176

   Plasmid

   #53351

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1039-1176

   Promoter

   tac

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. GST-PRDM16 681-880

   Plasmid

   #53349

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 681-880

   Promoter

   tac

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. GST-PRDM16 455-680

   Plasmid

   #53348

   Purpose

   bacterial expression of mouse PRDM16 fragment

   Depositor

   Shingo Kajimura, Bruce Spiegelman

   Article

   Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 455-680

   Promoter

   tac

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pKS13

   Plasmid

   #24627

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   pdc, adhB
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pRSETa-mEos3.2-A69T

   Plasmid

   #98571

   Purpose

   photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos3.2-A69T
   

   Tags

   7xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCoofy16

   Plasmid

   #43988

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His10-NusA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

   Plasmid

   #106464

   Purpose

   Expresses Prc1 with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   PRC1
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   PRC1

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DNMT3B

   Plasmid

   #25163

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DNMT3B_18 (DNMT3B Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pET MBP mOrange LIC cloning vector (MBP-mOrange)

   Plasmid

   #29748

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

   Expression

   Bacterial

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB1-mEGFP

    Plasmid

    #103979

    Purpose

    Plasmid encoding mEGFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mEGFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-Ets1-ETS

    Plasmid

    #85735

    Purpose

    Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

    Depositor

    Gregory Poon

    Article

    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

    Insert

    DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)
    

    Tags

    6xHis following thrombin cleavage site LVPR|G

    Expression

    Bacterial

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-Casp8 delta DED C360A

    Plasmid

    #11828

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Minus Death Defecter Domain (aa 1-216), C360A

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pBW213ara-hrpS

    Plasmid

    #61435

    Purpose

    pBAD18-cm encoding rbs33-hrpS-ter

    Depositor

    Martin Buck, Baojun Wang

    Article

    Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

    Insert

    rbs33-hrpS-ter
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. B6-4

    Plasmid

    #111488

    Purpose

    Phe-tRNA synthetase A

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Phe-tRNA synthetase A
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A81S

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXA5 (human) HIS-tag pET

    Plasmid

    #8562

    Depositor

    Corey Largman

    Insert

    HOXA5 (HOXA5 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    SacI site at amino acids 1-2 was used to clone in…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid

    #205021

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT-mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW4.16

    Plasmid

    #62871

    Purpose

    Bacterial expression of MBP-MBK-2

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MBK-2
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-51b(+) ChemoG1

    Plasmid

    #193799

    Purpose

    Protein production of ChemoG1 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG1
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-51b(+) ChemoG2

    Plasmid

    #193800

    Purpose

    Protein production of ChemoG2 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG2
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP: A206K

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-51b(+) ChemoG3

    Plasmid

    #193801

    Purpose

    Protein production of ChemoG3 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG3
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K; HT7: L271E

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge