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pLPC32

Plasmid

#209997

Purpose

Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

3' Connector (3C5CSN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC52

Plasmid

#209991

Purpose

Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

5' Connector (5C2SN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC51

Plasmid

#209990

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

5' Connector (5C1CSN)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1_LIM

Plasmid

#109780

Purpose

Protein expression and purification of LASP1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LASP1_LIM (LASP1 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Tags

His and S

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pTC-mcs

Plasmid

#20317

Depositor

Dirk Schnappinger

Article

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

multiple cloning site

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJMD13

Plasmid

#138653

Purpose

phiC31 integrating Streptomyces expression vector

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Tags

StrepII

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pdCas9-M-3A2

Plasmid

#73435

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor 3A2 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive wild-type S. pyogenes promoter

Available Since

March 3, 2016

Availability

Academic Institutions and Nonprofits only

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ELK4_pet28a

Plasmid

#131648

Purpose

Bacterial expression of ETS gene ELK4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK4 (ELK4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

Plasmid

#217767

Purpose

Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Protein purification (6xhis tag, tev site, sumo3 …

Tags

sfGFP

Expression

Bacterial

Mutation

CA3 domain aa 317-400

Promoter

T7 promoter

Available Since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

Plasmid

#217766

Purpose

Fluorescent reporter for ceramide (for bacterial expression and purification)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Protein purification (6xhis tag, tev site, sumo3 …

Tags

sfGFP

Expression

Bacterial

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

T7 promoter

Available Since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSC101-GFPmut2-mScarlet-I TAG

Plasmid

#208188

Purpose

nonsense mistranslation, TAG stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAG*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGA

Plasmid

#208189

Purpose

nonsense mistranslation, TGA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGATC

Plasmid

#208190

Purpose

nonsense mistranslation, TGA stop variant 2

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7TCT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I T7

Plasmid

#208191

Purpose

frameshift mistranslation, T7

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6TTTTTTT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A5

Plasmid

#208192

Purpose

frameshift mistranslation, A5

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A4

Plasmid

#208193

Purpose

frameshift mistranslation, A4

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A6T

Plasmid

#208194

Purpose

frameshift mistranslation, A6T

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAAAT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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