32,069 results
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Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046758
Plasmid#145670PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
RAB30
Plasmid#25542PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRExpressionBacterialAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
ExpressionBacterialAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BASIC_11_RBS34-GFP-B0015
Plasmid#68145Purposestorage vector for BASIC part: RBS-GFP(ORF)DepositorInsertRBS-34-GFP-B0015
ExpressionBacterialAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTCF176 HLA-A*02:01 (CD8 mut)
Plasmid#180459PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01 (CD8 mut)
TagsBSP41ExpressionBacterialMutationD227K, T228A, replacement of transmembrane domain…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-TTlight-4xDQNAT
Plasmid#128402PurposePeriplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertTetanus toxin light chain domain
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating E234A mutationPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B
Plasmid#67334PurposehArl8b with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 NLS-GFP
Plasmid#129441Purposebacterial expression of GFPDepositorInsertGFP
ExpressionBacterialAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Delta DED FLIP-L
Plasmid#13790DepositorInsertDELTA DED FLIP LONG (CFLAR Human)
Tags8 x His tagExpressionBacterialMutationDelta DED removes first 186 residuesAvailable SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
HOXA5 (human) HIS-tag pET
Plasmid#8562DepositorInsertHOXA5 (HOXA5 Human)
TagsHis and T7ExpressionBacterialMutationSacI site at amino acids 1-2 was used to clone in…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WT)
Plasmid#111323Purposefor T7 transcription of wt actin pre-mRNADepositorInsertACT1
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204794PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
ExpressionBacterialAvailable SinceAug. 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad-HaloGFP-Ca0.1(Na0.1, 143-144)
Plasmid#198599PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-143-144
ExpressionBacterialPromoteraraBAD promoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA4-RFP
Plasmid#62250Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA4Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP-NMT
Plasmid#25200PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLmjF32.0080:P5-L421
TagsHisExpressionBacterialAvailable SinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIA1364
Plasmid#172401PurposeExpression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -