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pET29b-EPN01*-His

Plasmid

#84509

Purpose

E.coli version of EPN01*

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01*-His_pET29b

Tags

His tag

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYB-Dual(mNG(yeast-opt))

Plasmid

#216727

Purpose

Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

Depositor

Emil Jensen

Article

Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

Insert

mNeonGreen

Expression

Bacterial and Yeast

Promoter

pGAL1(yeast) and pT7-RNAP(bacteria)

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-mMcl-1

Plasmid

#25717

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (Mcl1 Mouse)

Tags

Gst

Expression

Bacterial

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

Plasmid

#119059

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Expression

Bacterial

Promoter

E1b minimal promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GST TopBP1 D (aa 1-1013)

Plasmid

#20374

Depositor

Aziz Sancar

Article

Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

Insert

TopBP1 (1-1013) (TOPBP1 Human)

Expression

Bacterial

Mutation

amino acids 1-1013

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-MK3

Plasmid

#30188

Depositor

Bruce Tempel

Insert

Kcna3 (Kcna3 Mouse)

Expression

Bacterial

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
PTPRN2

Plasmid

#38891

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPRN2 (PTPRN2 Human)

Tags

His

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
GST-GIV-CT

Plasmid

#69863

Purpose

Bacterial expression of GST tagged GIV-CT

Depositor

Pradipta Ghosh

Article

Garcia-Marcos et al J Biol Chem. 2010 Apr 23;285(17):12765-7

Insert

GIV/Girdin (CCDC88A Human)

Tags

GST

Expression

Bacterial

Mutation

C terminal amino acids 1623-1870 of human GIV

Promoter

TAC

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and Citrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pIB169

Plasmid

#90192

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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GST-U1C

Plasmid

#1840

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

U1C (SNRPC Human)

Tags

GST

Expression

Bacterial

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
PDLIM7

Plasmid

#38878

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PDLIM7 (PDLIM7 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBAT4

Plasmid

#112580

Purpose

Used for expression of protein in E. coli as non-fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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GST-FUS 356-526

Plasmid

#44982

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (356-526aa) (FUS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 356-526

Promoter

tac

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pSF2C-A-RFP

Plasmid

#172432

Purpose

StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Type

Empty backbone

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Bluescript-OPNb

Plasmid

#107979

Purpose

expression of OPNb

Depositor

Georg Weber

Insert

osteopontin (OPNb)

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript PMS1

Plasmid

#16457

Depositor

Bert Vogelstein

Article

Nicolaides et al Nature. 1994 Sep 1. 371(6492):75-80.

Insert

PMS1 (PMS1 Human)

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only