30,307 results

1. pASKt15C+SrtA

   Plasmid

   #64983

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQLinkHD_EBFP2

   Plasmid

   #118853

   Purpose

   fluorescent protein expression with an N-terminal His-tag

   Depositor

   Marc Birtwistle

   Article

   Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

   Insert

   EBFP2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   EBFP1.2-I128V/V150I/ D155V/V224R

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-Squ:mCherry

   Plasmid

   #20163

   Depositor

   Eric Wieschaus

   Article

   Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

   Insert

   spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. HOXA1 (murine) HIS-tag pET

   Plasmid

   #8556

   Depositor

   Corey Largman

   Insert

   HOXA1 (Hoxa1 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   clone contains 130 AA including the homeodomain, …

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pET21a - GR

   Plasmid

   #89614

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Glutaredoxin
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAW204

   Plasmid

   #113244

   Purpose

   E. coli expression vector for IHFa F13A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa F13A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F13A

   Promoter

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 21 pCOLI_A_N-GST_C_STREP

   Plasmid

   #160258

   Purpose

   N-term GST 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   2xStrepII and GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX Tyk2-SH2

   Plasmid

   #46527

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Tyk2 (TYK2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 452-567

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbB2c-GFP

   Plasmid

   #35344

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPSP6

    Plasmid

    #48150

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-sfGFP[D134TAG+N150TAA]

    Plasmid

    #164582

    Purpose

    superfolder GFP with D134TAG and N150TAA mutations

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-134TAG+150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAA Mutations

    Promoter

    T7

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZa-tetO-csgAW-CmR

    Plasmid

    #98937

    Purpose

    Conductive Curli Protein csgAW

    Depositor

    Urartu Seker

    Article

    Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

    Insert

    csgAW
    

    Use

    Tags

    WWRKWKEWDDW

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZa-tetO-csgAY-CmR

    Plasmid

    #98938

    Purpose

    Conductive Curli Protein csgAY

    Depositor

    Urartu Seker

    Article

    Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

    Insert

    csgAY
    

    Use

    Tags

    YYRKYKEYDDY

    Expression

    Bacterial

    Mutation

    Promoter

    tetO

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS Wnt-4HA

    Plasmid

    #17375

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-4HA (Wnt4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pEPYCdKN0001

    Plasmid

    #196148

    Purpose

    Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit

    Depositor

    Nicola Patron

    Article

    Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

    Insert

    9xHis_AtTGA1_HiBit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6559 pJC25YDJ1 #7

    Plasmid

    #1245

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Ydj1 (YDJ1 Budding Yeast)
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGEX Vav2-SH2

    Plasmid

    #46529

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 665-774

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1

    Plasmid

    #81060

    Purpose

    mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    VAV2 DH/PH/C1 (VAV2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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