29,813 results

1. pRF-SmcR

   Plasmid

   #49371

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   SmcR repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETNKI-strepII-3C-LIC-kan

   Plasmid

   #108706

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEHIShqprt

   Plasmid

   #92166

   Purpose

   Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

   Depositor

   Huanting Liu, James Naismith

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   Quinolinate Phosphoribosyltransferase (QPRT Human)
   

   Use

   Tags

   TEV protease -cleavable N-terminal 6His

   Expression

   Bacterial

   Mutation

   changed Asp 2 to Asn to create BspHI site

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFTK019

   Plasmid

   #171291

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PTET-10x-repeat promoter+CPgpdA (fused)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFTK017

   Plasmid

   #171289

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PTET-5x-repeat promoter+CPgpdA (fused)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFTK038

   Plasmid

   #171310

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   hph, hygR, hygromycin selection marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFTK037

   Plasmid

   #171309

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   bleoR phleomycin, selection marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJRTCLCrVA010

   Plasmid

   #22843

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA containing GFP
   

   Use

   Cotton expression vector

   Tags

   Expression

   Bacterial

   Mutation

   Cotton Leaf Crumple Virus A component with Coat P…

   Promoter

   Available since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJET-07-mNeonGreen-R11a

   Plasmid

   #179890

   Purpose

   Donor with homologous arms for Rab11a to knock in mNeonGreen

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. cp193g7N

    Plasmid

    #25982

    Depositor

    Robert Campbell

    Article

    Carlson et al Protein Sci. 2010 Jun 2. ():.

    Insert

    cp193g7N
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pST50Trc4-STRNDHFR

    Plasmid

    #63996

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DAXX-myc

    Plasmid

    #1851

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    DAXX (Daxx Mouse)
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pTHSSd_38

    Plasmid

    #59961

    Purpose

    High core fragment expression (high resource allocator)

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    core fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AP682-1

    Plasmid

    #70050

    Purpose

    Expresses TEV::eGFP::myc::3Xflag in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    eGFP
    

    Use

    Tags

    TEV and myc::3Xflag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMMRSF17

    Plasmid

    #20178

    Depositor

    Hideo Iwai

    Article

    Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

    Insert

    C-CrkII nSH3 (CRK Human)
    

    Use

    Tags

    NpuNdelC16 and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ACOT12BA

    Plasmid

    #42395

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACOT12BA (ACOT12 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. PBRM1

    Plasmid

    #39103

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

    Plasmid

    #99342

    Purpose

    Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

    Depositor

    Peter Wright

    Article

    Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

    Inserts

    STAT1 (710-750) (STAT1 Human)
    CBP (1764-1855)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBluescript SK - hTRF1

    Plasmid

    #12306

    Depositor

    Titia de Lange

    Article

    Chong et al Science. 1995 Dec 8. 270(5242):1663-7.

    Insert

    hTRF1 (TERF1P3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only