30,307 results

1. pBAD-HisD-mNG-D-Kbp

   Plasmid

   #178012

   Purpose

   Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   mNG-D-Kbp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   Dec. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX 4t1 RARalpha (1-187)

   Plasmid

   #35558

   Depositor

   Harish Srinivas, Jonathan Kurie

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1-187

   Promoter

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

3. PGEX-6P1-hNF2-AR mutant

   Plasmid

   #107149

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

   Insert

   neurofibromin 2 (NF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   A585W-R588K

   Promoter

   Ptac

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbE5c-RFP

   Plasmid

   #35278

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZS1[TreR-T,LacI-T]

   Plasmid

   #60770

   Purpose

   Contains PI driving expression TreR-T, and PI driving expression of LacI-T.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   TreR-T
   Dimeric LacI with the TAN DBD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Dimeric LacI with the TAN DBD. and LacI/GalR repr…

   Promoter

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pND004

   Plasmid

   #200950

   Purpose

   MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTETDuet_WTDHFR_WTTS

   Plasmid

   #191282

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT DHFR, WT TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18-iSpinach

   Plasmid

   #186053

   Purpose

   template for co-transcriptional RNA folding assays

   Depositor

   Markus Wahl

   Article

   Huang et al Mol Cell. 2020 Sep 17;79(6):1024-1036.e5

   Insert

   T7A1-rrnG-iSpinach
   

   Use

   Tags

   no

   Expression

   Bacterial

   Mutation

   Promoter

   T7A1

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Clim-DC2-NMM-tdtomato

   Plasmid

   #129557

   Purpose

   pClimDC-NMM:tdT cell membrane marker

   Depositor

   Inaki Ruiz-Trillo

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   N-Myristoylation motif (NMM) fused to tdtomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSM12

    Plasmid

    #101643

    Purpose

    Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    meffCFP
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ZP9 Actin

    Plasmid

    #16932

    Depositor

    Randall Moon

    Insert

    Beta-actin (actb1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2008

    Availability

    Academic Institutions and Nonprofits only

12. CNNM3-CBS-YPet

    Plasmid

    #149686

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CNNM3-CBS-YPet (CNNM3 Synthetic, Human)
    

    Use

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PBRM1

    Plasmid

    #39013

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pBabe-PI(WT)-Rac1(WT)-mCherry

    Plasmid

    #87550

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b-NpR5599

    Plasmid

    #45740

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    NpR5599 (Npun_R5599 Nostoc punctiforme)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET32a-Trx-PP-hNF2

    Plasmid

    #107148

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    (His)6-Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His6 StrepII TEV co-transformation cloning vector (13K-HR)

    Plasmid

    #48318

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDM_noro_S2_lacZA

    Plasmid

    #118816

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQStrep2-GADD45G

    Plasmid

    #31584

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    growth arrest and DNA-damage-inducible, gamma (GADD45G Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge