30,695 results

1. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC-ZB-SPRTN-E112A-FL

   Plasmid

   #110218

   Purpose

   Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Tags

   His6 and ZB

   Expression

   Bacterial

   Mutation

   E112A, P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Smad3 LC

   Plasmid

   #12633

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

   Insert

   Smad3 LC (SMAD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LC segment of Smad3 (aa 146-425)

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pMtb CblA

   Plasmid

   #138298

   Purpose

   Expression of Mtb CblA

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Insert

   Mtb CblA
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMtb ATR

   Plasmid

   #138297

   Purpose

   Expression of Mtb ATR

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Insert

   Corrinoid adenosyl transferase
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-SUMO-KaiA

   Plasmid

   #68049

   Purpose

   Strain: AM5115, Used for protein purification

   Depositor

   Susan Golden

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

   Insert

   KaiA
   

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nsp3 Mac1 - C2 (UCSF)

   Plasmid

   #169209

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pHH0103_ABL1-1/1

   Plasmid

   #91521

   Purpose

   Protein expression and purification of human SH3 domain construct ABL1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ABL1-1/1 (ABL1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDKL1_HUMAN_D0

   Plasmid

   #79699

   Purpose

   This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CDKL1 (CDKL1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1MycHis/HIP1/ΔE

    Plasmid

    #31252

    Depositor

    Theodora Ross

    Article

    Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

    Insert

    Huntingtin-interacting protein 1 (HIP1 Human)
    

    Tags

    his and myc

    Expression

    Bacterial

    Mutation

    deleted ENTH domain

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

12. GPX1

    Plasmid

    #38864

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GPX1 (Gpx1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    SeCys49 -> Gly

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pJRTCLCrVB1.3

    Plasmid

    #22844

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    B DNA
    

    Use

    Cotton vigs vector

    Expression

    Bacterial

    Mutation

    1.3 unit B componet length inserted into pBlueScr…

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1-comCerulean3

    Plasmid

    #104017

    Purpose

    Plasmid encoding comCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    comCerulean3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Same protein sequence as mCerulean3 (codon optimi…

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDUET-aaCPRct-A13AMO

    Plasmid

    #20116

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pBTB-1

    Plasmid

    #22817

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a MDH_PFALCI

    Plasmid

    #204235

    Purpose

    Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSW002-PpsbA-mNeonGreen

    Plasmid

    #205017

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEL2836

    Plasmid

    #201997

    Purpose

    Complementation of M. smegmatis LucB(WT)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

    Insert

    lucB
    

    Expression

    Bacterial

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIC-Bio2_SMAC(delta(55))_9His

    Plasmid

    #197976

    Purpose

    E. coli expression of SMAC(delta(55))-9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55)

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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