31,188 results
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Plasmid#160086PurposeBacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKADepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
5KO9 (HMCES)
Plasmid#101877PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHMCES (HMCES Human)
Tags6x His (modified C-term tag is AENLYFQSHHHHHH)ExpressionBacterialPromoterT7Available SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_RANBP2_Ran-BP2
Plasmid#109839PurposeProtein expression and purification of RANBP2_Ran-BP2DepositorInsertRANBP2_Ran-BP2 (RANBP2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRelA'
Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
ExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2delBRCT
Plasmid#173939PurposeBacterial expression of PARP1 lacking Zn2 and BRCT domainsDepositorInsertPARP1delZn2delBRCT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 and 374-520; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK556
Plasmid#74277PurposeProduces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase
TagsHis6ExpressionBacterialMutationchanges alanine-1246 to serine, numerous silent m…PromoterT7Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only