30,260 results

1. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. IL-4 promoter luciferase

   Plasmid

   #12195

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-4 promoter (Il4 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-uvsX-H6

   Plasmid

   #163913

   Purpose

   Expresses uvsX for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsX
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM33_SUFU_Sufu

   Plasmid

   #178488

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SUFU_Sufu (SUFU Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-GFPplus

   Plasmid

   #172718

   Purpose

   Expression of a fast folding GFP variant GFP plus

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   GFP plus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIRT5-pET15b

   Plasmid

   #81030

   Purpose

   Bacterial expression of Human WT SIRT5 protein

   Depositor

   David Lombard

   Article

   Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

   Insert

   Sirt5 (SIRT5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pnEA-vH_His-TEV-SEPT2

   Plasmid

   #174491

   Purpose

   bacterial expression of human SEPT2

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

   Plasmid

   #61590

   Purpose

   Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

   Depositor

   David Komander

   Article

   Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-244 and aa 698-708.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET15b-hnRNP-H

    Plasmid

    #23020

    Depositor

    Douglas Black

    Article

    Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

    Insert

    hnRNP-H (HNRNPH1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR-spondin 1

    Plasmid

    #128844

    Purpose

    bacterial expression of pR-spondin 1

    Depositor

    Marc de la Roche

    Article

    Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

    Insert

    R-spondin 1 (RSPO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBad-CA

    Plasmid

    #105665

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-mhYFP

    Plasmid

    #186532

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMLRT

    Plasmid

    #78234

    Purpose

    Expresses Murine Leukemia Reverse Transcriptase (MLRT)

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Insert

    Murine leukemia reverse transcriptase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET-MagLOV

    Plasmid

    #219957

    Purpose

    Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

    Depositor

    Andrew York

    Article

    Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

    Insert

    MagLOV
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    AsLOV2 C450P L496V Q513K G528K D540M

    Promoter

    T7

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPROEX-TwStrp-FTO

    Plasmid

    #190265

    Purpose

    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.

    Depositor

    Samie Jaffrey

    Insert

    FTO (FTO Human)
    

    Use

    Tags

    6xHis and Twin-Streptag

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rb-379-792-pGex2TK

    Plasmid

    #21748

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-792 of wt Rb …

    Promoter

    Available since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge