30,737 results

1. pMyC

   Plasmid

   #42192

   Depositor

   Matthias Wilmanns, Annabel Parret

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Promoter

   ColE1

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE31-rsEGFP2

   Plasmid

   #102879

   Purpose

   Expresses rsEGFP2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

   Insert

   rsEGFP2
   

   Tags

   6xHisTag

   Expression

   Bacterial

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET.HaloTag.v7

   Plasmid

   #226197

   Purpose

   bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Non tagged Avidin

   Plasmid

   #214835

   Purpose

   This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   Non tagged Avidin
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-GlmU-NahK-ET64

   Plasmid

   #234663

   Purpose

   Expression an ELP-tagged fusion enzyme of GlmU and NahK

   Depositor

   Xianwei Liu

   Article

   Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

   Inserts

   N-acetylglucosamine- 1-phosphate uridyltransferase
   N-acetylhexosamine 1-kinase
   

   Tags

   ELP

   Expression

   Bacterial

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgRNAtet-lvaA

   Plasmid

   #106397

   Purpose

   Guide RNA plasmid targeting lvaA on BBR1-UP origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   sgRNA towards lvaA in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS mStrawberry

   Plasmid

   #91768

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Strawberry
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad33_ISDge10_reRNA

    Plasmid

    #205340

    Purpose

    Bacterial expression of ISDge10 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISDge10 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5371

    Plasmid

    #128732

    Purpose

    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

    Depositor

    Alan Collmer

    Article

    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET3a-OSF-CypA

    Plasmid

    #79039

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pABE8e-protein

    Plasmid

    #161788

    Purpose

    Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

    Depositor

    David Liu

    Article

    Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

    Insert

    ABE8e base editor
    

    Tags

    8xHIS and BPNLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCF525-EF1a-Hygro-P2A-mCherry-lenti

    Plasmid

    #115796

    Purpose

    Expresses mCherry in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1a promoter

    Available Since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xHis-MBP-huMb2Cas12a

    Plasmid

    #174676

    Purpose

    Expresses 6xHis-MBP tagged Mb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNitET-SacB-kan

    Plasmid

    #107692

    Purpose

    Expression of the phage Che9c RecET system for Recombineering in mycobacteria

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Inserts

    Che9 phage RecET
    PNit2-NitR
    SacRB
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX iLOV

    Plasmid

    #26587

    Depositor

    John Christie

    Article

    Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

    Insert

    iLOV
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-HTNC

    Plasmid

    #13763

    Purpose

    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

    Depositor

    Klaus Rajewsky

    Article

    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

    Insert

    His-TAT-NLS-Cre
    

    Use

    Baculovirus

    Tags

    His Tag, NLS, and TAT

    Expression

    Bacterial

    Promoter

    p10 promoter

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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