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Showing: 1481 - 1500 of 29690 results

  1. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid
    #171660
    Purpose
    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    Use
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171660-plasmid-map-sequence-id-339032

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  2. T7-LacO-QF-T7-TetO-TetR_p15A

    Plasmid
    #171661
    Purpose
    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QF-T7-TetO-TetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171661-plasmid-map-sequence-id-342537

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  5. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Use
    Tags
    Expression
    Bacterial and Worm
    Mutation
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

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  10. pSG058

    Plasmid
    #214261
    Purpose
    PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214261-plasmid-map-sequence-id-432467

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  11. pRS413-GAL1-yEGFP1

    Plasmid
    #40235
    Depositor
    Michael Benton
    Insert
    yEGFP1
    Use
    Tags
    HIS3MX6
    Expression
    Bacterial
    Mutation
    Promoter
    GAL1
    Available since
    Oct. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    40235-plasmid-map-sequence-id-51356

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  12. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  15. pBAD-bARGSer-AxeTxe

    Plasmid
    #192473
    Purpose
    Second-generation bacterial acoustic reporter gene derived from Serratia
    Depositor
    Mikhail Shapiro
    Article
    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Inserts
    bARGSer
    AxeTxe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    the gene Ser39006_001280 was deleted from the clu…
    Promoter
    Native promoter from Enterococcus faecium and pBAD
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    192473-plasmid-map-sequence-id-376957

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  16. pCDB179-DuraPETase

    Plasmid
    #218699
    Purpose
    Encodes codon optimized DuraPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    DuraPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218699-plasmid-map-sequence-id-434102

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  20. pM1s3ATScsg-Etag

    Plasmid
    #137946
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    Synthetic curli operon
    Synthetic curli operon
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTlpA36
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137946-plasmid-map-sequence-id-331396

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Showing: 1481 - 1500 of 29690 results