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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT31

Plasmid

#89666

Purpose

expression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT31

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT27

Plasmid

#89665

Purpose

expression of doubleTAL staple protein dT27 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT27

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBbE5a-RFP

Plasmid

#35277

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCK351

Plasmid

#129696

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120034435

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pKJ1267 (aka pAC-alphaGal4)

Plasmid

#21869

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_GAS7-1/1

Plasmid

#91301

Purpose

Protein expression and purification of human SH3 domain construct GAS7-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GAS7-1/1 (GAS7 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSd_41

Plasmid

#59964

Purpose

High full length T7 RNAP* expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7* RNAP

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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