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Poliovirus 1 PV1 isolate 09_05_19 3C protease

Plasmid

#228647

Purpose

Bacterial expression of the Polio 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Polio virus 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKJ0464

Plasmid

#228716

Purpose

Bacterial expression of T7 RNAP with E643G mutation; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

SUmo_T7pol_E643G

Expression

Bacterial

Mutation

E643G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHelper_AraC/pBad-Tnpase (camR)

Plasmid

#213909

Purpose

Arabinose-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

AraC/pBad ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJH628_pJH_LldR_PlldR-sfGFP.3TAG

Plasmid

#163727

Purpose

Lactate-inducible expression of green fluorescent protein encoding three amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.3TAG

Expression

Bacterial

Mutation

gfp.N39TAG,N135TAG,Y151TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-eyfp

Plasmid

#65039

Purpose

ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human full-length LIC1

Plasmid

#74597

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRAU168

Plasmid

#86834

Purpose

bacterial expression of anti-CRISPR protein AcrIIA1

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA1

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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