30,260 results

1. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB179-Z1-PETase

   Plasmid

   #218703

   Purpose

   Encodes codon optimized Z1-PETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   Z1-PETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBSV2G_PflaB-msfGFPBb

   Plasmid

   #118231

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-zDjCas13d-His

   Plasmid

   #234481

   Purpose

   Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DjCas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-HA-AR-NTD-DBD

   Plasmid

   #171236

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKO403_lacZ_Sp

   Plasmid

   #174726

   Purpose

   Bifidobacterium-E.coli shuttle vector for seamless gene cloning

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-K-GECO1

   Plasmid

   #105864

   Purpose

   Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Use

   Tags

   6-His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAF1

   Plasmid

   #39117

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TAF1 (TAF1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pSCrhaB2plus

    Plasmid

    #164226

    Purpose

    Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

    Depositor

    Silvia Cardona

    Article

    Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose-inducible

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-Lime

    Plasmid

    #191255

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein Lime

    Depositor

    Robert Campbell

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Lime
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL-pylT-AznLRS

    Plasmid

    #172482

    Purpose

    For E.Coli expression, 306L309L348I384F411W

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

    Insert

    AznLRS and pylTcua
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET20b-delta133HtrA2

    Plasmid

    #14126

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

    Plasmid

    #202025

    Purpose

    Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpB S9G-R31L-R85L

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-GINKO2

    Plasmid

    #177116

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    GINKO2
    

    Use

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACM4

    Plasmid

    #49797

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Use

    Tags

    S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-BirA-eCPX-AP

    Plasmid

    #121907

    Purpose

    Expresses BirA and eCPX-AP

    Depositor

    Per Svenningsen

    Article

    Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

    Inserts

    BirA (birA Synthetic)
    Enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and pBAD promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH6HTC_HSPA9-Halo

    Plasmid

    #175332

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HSPA9 (HSPA9 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

    Plasmid

    #231128

    Purpose

    Expresses xylanase via a cSAT2.0 scheme

    Depositor

    Xiaofeng Yang

    Article

    Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

    Insert

    xylanase
    

    Use

    Tags

    cSAT2.0

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid

    #86928

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and td…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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