30,416 results

1. pET21a-alpha-synuclein

   Plasmid

   #51486

   Purpose

   Inducible expression of human alpha-synuclein in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pPET-PKR/PPase

   Plasmid

   #42934

   Depositor

   James Cole

   Article

   Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

   Inserts

   protein kinase R (EIF2AK2 Human)
   phage lambda phosphatase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ClCry4 Y319D

   Plasmid

   #160649

   Purpose

   Expression of protein for various experiments including finding interaction partners

   Depositor

   Joseph Takahashi

   Article

   Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

   Insert

   Clcry4 PHR Y319D
   

   Use

   Tags

   Hexa his

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Use

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sumo3 PolQM1

   Plasmid

   #78462

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; Q2513R (please see depositor commen…

   Promoter

   T7

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQTEV-PFDN2

   Plasmid

   #31329

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   prefoldin 2 (PFDN2 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-HA-Tuba

    Plasmid

    #22214

    Depositor

    Pietro De Camilli

    Article

    Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

    Insert

    Tuba (DNMBP Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only