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pDEST14-SpyCatcher002 EQ

Plasmid

#102830

Purpose

Bacterial expression of unreactive version of SpyCatcher002

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002 EQ

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

E77Q mutation prevents isopeptide bond formation

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTK164

Plasmid

#13533

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTY

Tags

ECFP and Venus

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(D130N)-RagC

Plasmid

#99642

Purpose

Bacterial expression of RagA(D130N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D130N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAJM.716

Plasmid

#108518

Purpose

PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBAD-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDJSVT91

Plasmid

#109305

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

SufI 1-30, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) FLAG pSP65

Plasmid

#8537

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-mRFP1-kanMX6

Plasmid

#133551

Purpose

Yeast fluorescent (RFP) vector with PTEF promoter.

Depositor

Won-Ki Huh, Erin O'Shea

Article

Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ2-Pdadh-LacZ

Plasmid

#191618

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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6BYB (L3MBTL1)

Plasmid

#119957

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

L3MBTL1 (L3MBTL1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pACYC-PLlambda-alkS(WT)

Plasmid

#44382

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkS(WT)

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlambda

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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