30,260 results

1. pSAM_AraC

   Plasmid

   #91569

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

   Insert

   AraC/PBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad33_ISAam1_reRNA

   Plasmid

   #205322

   Purpose

   Bacterial expression of ISAam1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISAam1 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. RPO41

   Plasmid

   #205072

   Purpose

   RPO41 protein expression

   Depositor

   Smita Patel

   Article

   Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

   Insert

   RPO41 (RPO41 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks N Terminal 100 amino acids

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-CUL4A-pCMV7.1

   Plasmid

   #155022

   Purpose

   N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 4A (CUL4A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNP1723

   Plasmid

   #222727

   Purpose

   sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS-lpp0.2-K64A

   Plasmid

   #201789

   Purpose

   mutant of PadR; transcriptional regulator can respond to p-coumaric acid

   Depositor

   Yajun Yan

   Article

   Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

   Insert

   PadR (padR Bacillus subtilis)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K64A

   Promoter

   Available since

   July 12, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sigma 70 promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-mGreenLantern

    Plasmid

    #161913

    Purpose

    mGreenLantern fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2CT-His-MBP-Lwa_Cas13a_WT

    Plasmid

    #91865

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LwaCas13a
    

    Use

    CRISPR

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT-HA-hM4D(Gi)

    Plasmid

    #45548

    Purpose

    Expresses hM4D (Gi) neuronal inhibitor

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM4D (Gi)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET17_K560_GFP_His

    Plasmid

    #15219

    Depositor

    Ron Vale

    Article

    Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

    Insert

    Human neuronal kinesin heavy chain (KIF5B Human)
    

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET FLIPPi-30m

    Plasmid

    #13556

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi T22A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    T22A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P2-MDM2 (17-125)

    Plasmid

    #62063

    Purpose

    Expression of human MDM2 (17-125) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 17-125

    Promoter

    tac

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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