29,692 results

1. pAB094a

   Plasmid

   #79241

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4; PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIOM15

   Plasmid

   #20869

   Depositor

   Carmen Beuzón

   Article

   Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

   Insert

   aphA::aadA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pUCP20T-ecfp

   Plasmid

   #78463

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. IMS-APEX2

   Plasmid

   #79058

   Purpose

   Expresses APEX in the mitochondrial intermembrane space.

   Depositor

   Alice Ting

   Article

   Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

   Insert

   APEX
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF4Ag NanoLuc (ATG-42)

   Plasmid

   #137777

   Purpose

   Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

   Depositor

   Lance Encell

   Article

   Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

   Insert

   NanoLuc (Nluc)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   NanoLuc contains 16 amino acid changes compared t…

   Promoter

   T7 and CMV

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZac2.1 gfaABC1D-eGFP

   Plasmid

   #176861

   Purpose

   Expresses cytosolic eGFP in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKS17

   Plasmid

   #24628

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   pdc, adhB, atfA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. ELONGIN BC (XX01TCEB1A-c001)

   Plasmid

   #110274

   Purpose

   Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

   Depositor

   Nicola Burgess-Brown

   Insert

   Elongin BC (ELOC, ELOB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Elongin B is full length. Elongin C contains a.a…

   Promoter

   T7

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH1105

   Plasmid

   #131864

   Purpose

   Produces holo-PhyB-AviTag in E. coli

   Depositor

   Wilfried Weber

   Article

   Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

   Inserts

   PhyB(1-651)-AviTag-His6
   HO1
   PcyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter-1 and T7 promoter-2

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-HA-BI-AR

    Plasmid

    #171234

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ptac::ccdB

    Plasmid

    #15922

    Depositor

    Nina Papavasiliou

    Article

    Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

    Insert

    ccdB (ccdB E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD-LSSmOrange

    Plasmid

    #37129

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    araB

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFV2-HA

    Plasmid

    #187082

    Purpose

    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

    Depositor

    Steven Vik

    Article

    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

    Insert

    atpBEFHAGDC
    

    Use

    Tags

    6-His beta subunit (atpD) and HA tag subunit a (a…

    Expression

    Bacterial

    Mutation

    All Cys codons changed to Ala.

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBb(B5)1k-GFPuv

    Plasmid

    #106393

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on BBR1-B5 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ki67∆FHA-mNeonGreen-IRESpuro2

    Plasmid

    #183739

    Purpose

    Expresses Ki67∆FHA-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67_lacking FHA (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking FHA)

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMpGWB402-er-mCherry

    Plasmid

    #186288

    Purpose

    Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    er-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid

    #181743

    Purpose

    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    T7

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK092

    Plasmid

    #183146

    Purpose

    pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2Bc-T MBP_BoMoC(ed)_F753A_6xH

    Plasmid

    #185710

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed F753 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge