33,434 results
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Plasmid#182240PurposeTripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.DepositorInsertGFP1-9
Tags6HISExpressionBacterialPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF158 Human beta-2 microglobulin
Plasmid#180457PurposeE. coli expression of class I MHC light chainDepositorInsertHuman beta-2 microglobulin (B2M Human)
ExpressionBacterialAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
spNW100
Plasmid#173488PurposeExpresses spNW100 in Bl21DepositorInsertspNW100
TagsHistagExpressionBacterialMutationP1049L- please see depositor commentsPromoterT7Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL117
Plasmid#163635PurposeOptimized M. smegmatis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plRL19
Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
ExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-DogCatcher
Plasmid#171772PurposeExpresses DogCatcher protein in bacterial cytoplasmDepositorInsertDogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
ExpressionBacterialPromoterhsp60Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPP381
Plasmid#158798PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-2
Tags6xHisExpressionBacterialMutationRuvC-inactivating mutation D394AAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138131PurposeExpresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmCherry-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-loop
Plasmid#132695PurposepRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX8
Plasmid#127453PurposeEncodes MqsR toxin and tsPurple chromoprotein. CAM-RDepositorInsertsmqsR toxin
tsPurple chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterapFAB46 and pRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyExpressionBacterialPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
ExpressionBacterialAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MCM3/5
Plasmid#116950PurposeExpresses human MCM3 and human MCM5DepositorAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-132
Plasmid#118550PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-FRB-PafA
Plasmid#113404PurposeExpresses FRB-PafA in bacteriaDepositorInsertFRB-PafA
TagsGSTExpressionBacterialPromotertacAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-BCCP-PupE
Plasmid#113403PurposeExpresses BCCP-PupE in bacteriaDepositorInsertBCCP-PupE
TagsGSTExpressionBacterialPromotertacAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by