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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-XI

Plasmid

#157988

Purpose

Expresses XI in E.coli cell

Depositor

Byung Il Lee

Insert

xylose isomerase

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_NoMTS_L6_pET28a+

Plasmid

#60012

Purpose

Expresses TFAM No MTS L6 mutant in bacterial cells

Depositor

David Chan

Article

Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

Insert

Human TFAM No MTS (TFAM Human)

Tags

6xHis

Expression

Bacterial

Mutation

Missing 1st 42 aas (Mitochondrial targeting seque…

Promoter

T7

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p2H12Matry

Plasmid

#221143

Purpose

cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry

Expression

Bacterial

Promoter

Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV E

Plasmid

#145361

Purpose

Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d_RspCasWYL1

Plasmid

#108304

Purpose

Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Inserts

RspCas13d
RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1

Plasmid

#194645

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMflT-o4

Plasmid

#101312

Purpose

Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pH6HTC_RAN-Halo

Plasmid

#175339

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

RAN (RAN Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP1346

Plasmid

#119274

Purpose

knockdown folA in K. pneumoniae

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA folA (K. pneumoniae)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-mCherry-Ki67

Plasmid

#183750

Purpose

Expresses EGFP-mCherry-Ki67

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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HUWE1

Plasmid

#25211

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

huwe1.4005.4374 (HUWE1 Human)

Tags

His

Expression

Bacterial

Mutation

HECT domain

Available Since

Feb. 18, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pUC18 mouse rDNA R1

Plasmid

#240559

Purpose

An rDNA FISH probe contains 10.7 kb fragment that spans the 3′ end of the intergenic spacer (IGS) and the beginning of the external transcribed spacer (ETS).

Depositor

Brian McStay, Shan Zha

Insert

Partial mouse rDNA sequence

Expression

Bacterial

Promoter

lac

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_His-TEV-SpyCatcher003-C

Plasmid

#233339

Purpose

To express SpyCatcher

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

His-TEV-SpyCatcher003-C

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Cre-LPETG-6xHis

Plasmid

#237224

Purpose

Expresses LPETG-tagged Cre recombinase in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Cre-LPETG (cre Synthetic)

Tags

6xHis

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 HA PTPL1

Plasmid

#237335

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

PTPL1 (PTPN13 Human)

Tags

HA

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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MAC3-N-GFP

Plasmid

#222602

Purpose

Expression of MAC3-tagged GFP (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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