We narrowed to 90 results for: Gileadi

1. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. XX01CAPN1A-c001

   Plasmid

   #175491

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN1 / CAPNS1 (CAPN1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. XX01CAPN2A-c001

   Plasmid

   #175492

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN2 / CAPNS1 (CAPN2 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIC-CTHF

   Plasmid

   #26105

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   TEV - His6 - Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PRDX1A-c001

   Plasmid

   #175473

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX1 (PRDX1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. COVID-SARS2 NSP13

   Plasmid

   #159614

   Purpose

   Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

   Depositor

   Opher Gileadi

   Article

   Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

   Insert

   Covid-SARS2 Nsp13
   

   Use

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   T7 - LacO

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. COVID-SARS2 NSP14/10

   Plasmid

   #159613

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

   Depositor

   Opher Gileadi

   Article

   Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

   Inserts

   Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
   Covid-SARS2 Nsp10
   

   Use

   Tags

   His6, TEV cleavage site and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PRDX6A-c001

   Plasmid

   #175474

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX6 (PRDX6 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CNN3A-c002

    Plasmid

    #175456

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-Y329.

    Depositor

    Opher Gileadi

    Insert

    CNN3 (CNN3 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SNX32A-c014

    Plasmid

    #175486

    Purpose

    Protein expression in bacterial cells. PX domain, S17-M197

    Depositor

    Opher Gileadi

    Insert

    SNX32 (SNX32 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNIC-ZB

    Plasmid

    #26107

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    His6 - Z-basic - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RAB5AA-c003

    Plasmid

    #175475

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-N215.

    Depositor

    Opher Gileadi

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIC-CH

    Plasmid

    #26117

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SYKA-c020

    Plasmid

    #175489

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EPB41L3A-c000

    Plasmid

    #175458

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-D1087.

    Depositor

    Opher Gileadi

    Insert

    EPB41L3 (EPB41L3 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TBXTA-c028

    Plasmid

    #139765

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, Full-length, WT (TBXT Human)
    

    Use

    Tags

    Avi-tag (Biotin) and His6-GST-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids S2- M435

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TBXTA-c006

    Plasmid

    #139755

    Purpose

    For protein expression/crystallography (PDB:6F59)

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-S224

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TBXTA-c043

    Plasmid

    #139766

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Use

    Tags

    Avi-tag (Biotin) and His6-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41- D225

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge