4,908 results

1. pLIB-GST-3C-CDK1

   Plasmid

   #177011

   Purpose

   Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Tags

   GST-3C

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-BbsI-chiRNA

   Plasmid

   #45946

   Purpose

   Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

   Depositor

   Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   U6-BbsI-chiRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Dm-snRNA:U6:96Ab

   Available since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-HIS-TEV-scCAK1

   Plasmid

   #177014

   Purpose

   Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIB-HIS-TEV-Cyclin-B

   Plasmid

   #177012

   Purpose

   Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBPLexA::p65Uw

   Plasmid

   #26231

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   LexA::p65 (RELA Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC19-13XLexAop2-IVS-myr::GFP

   Plasmid

   #26224

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   myr::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAEL010097-Cas9

   Plasmid

   #100707

   Purpose

   The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL010097-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL010097 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBPnlsLexA::GADflUw

   Plasmid

   #26232

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::GADfl
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcGP67A-mMD2-proA

   Plasmid

   #187878

   Purpose

   baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

   Depositor

   Bruce Beutler

   Article

   Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   MD2 (Ly96 Mouse)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   includes residues 19–160 of mouse MD-2

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

    Plasmid

    #71805

    Purpose

    Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRE-Hsp70BbCas9_1.3

    Plasmid

    #190797

    Purpose

    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJFRC7-20XUAS-IVS-mCD8::GFP

    Plasmid

    #26220

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR BirA

    Plasmid

    #113640

    Purpose

    Insect cell expression vector for BirA Biotin Ligase

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    BirA Biotin Ligase (birA )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218863

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACE-GIRK2-S

    Plasmid

    #172426

    Purpose

    Expression of full-length, human GIRK2 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-Nucleocapsid

    Plasmid

    #179696

    Purpose

    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

    Depositor

    Mark Arbing

    Insert

    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His6-TEV protease site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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