4,931 results

1. pFast-dCry-V5His

   Plasmid

   #136240

   Purpose

   Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

   Depositor

   Aziz Sancar

   Article

   Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

   Insert

   DmCry
   

   Tags

   His and V5

   Expression

   Insect

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. DYNLL2

   Plasmid

   #132531

   Purpose

   Human LC8-2, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLL2 (DYNLL2 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DYNLT3

   Plasmid

   #132532

   Purpose

   Human TCTEX-3, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLT3 (DYNLT3 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LC8-like

   Plasmid

   #133115

   Purpose

   Human LC8-like protein, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   LC8-like (LOC392067 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DYNLRB2

   Plasmid

   #132534

   Purpose

   Human Rbl-2, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLRB2 (DYNLRB2 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAcGP67B human Fzd4 CRD (38–160)

   Plasmid

   #132621

   Purpose

   expresses human Fzd4 CRD (38-160) in Hi5 insect cell

   Depositor

   Wenqing Xu

   Article

   Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

   Insert

   Fzd4 CRD (38–160) (FZD4 Human)
   

   Expression

   Insect

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hy_pCG6770 eGFP SV40

   Plasmid

   #132649

   Purpose

   Expresses eGFP mRNA from the Drosophila CG6770 promoter

   Depositor

   Jeremy Wilusz

   Article

   Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   CG6770

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Hy_ppst eGFP SV40

    Plasmid

    #132648

    Purpose

    Expresses eGFP mRNA from the Drosophila pst promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    pst

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Hy_pSirup eGFP SV40

    Plasmid

    #132647

    Purpose

    Expresses eGFP mRNA from the Drosophila Sirup promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    Sirup

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pCG8620 eGFP SV40

    Plasmid

    #132646

    Purpose

    Expresses eGFP mRNA from the Drosophila CG8620 promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    Hml

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Hy_pMtnA UTR delta 21-55 eGFP SV40

    Plasmid

    #132653

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    MtnA

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Hy_pMtnA UTR delta 41-55 eGFP SV40

    Plasmid

    #132652

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    MtnA

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Hy_pMtnA UTR 1-55 eGFP SV40

    Plasmid

    #132651

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 55 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    MtnA

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Hy_pHml eGFP SV40

    Plasmid

    #132645

    Purpose

    Expresses eGFP mRNA from the Drosophila Hml promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    Hml

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Hy_pMtnA eGFP MALAT1

    Plasmid

    #132643

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that ends in the MALAT1 triple helix

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    MtnA

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUAS attB V5 dDispatched WT HA

    Plasmid

    #126411

    Purpose

    Expression of wild type dDispatched HA in Drosophila.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    d-Dispatched1 (disp Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIE-N-hα2M-H6

    Plasmid

    #128493

    Purpose

    Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (S24-E908) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Insect

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

    Plasmid

    #124068

    Purpose

    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Article

    He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1

    Insert

    eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
    

    Use

    CRISPR and Cre/Lox

    Expression

    Insect

    Promoter

    bhsp68

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUAS-attB V5 dDispatched delta CS HA

    Plasmid

    #126412

    Purpose

    Expression of V5 dDispatched Delta CS HA in Drosophila cells lines

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    d-Dispatched1 (disp Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    Disp1 Cleavage site

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGS-BacA-21222-FLP-3xFLAG

    Plasmid

    #109165

    Purpose

    Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

    Insert

    Flp
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EBP50-pDONR221

    Plasmid

    #124243

    Purpose

    Gateway entry clone of Drosophila EBP50 cDNA

    Depositor

    Peter Vilmos

    Insert

    Drosophila EBP50 (CG10939 Fly)
    

    Expression

    Insect

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBac(3xP3-gTc’v; Valcyrie.Uni)

    Plasmid

    #119068

    Purpose

    Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (low)

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2019 Jan 4;146(1). pii: dev

    Inserts

    pBacL
    Tc-hsp68 Promoter
    NLS-Cas9
    Tc-hsp68 3'UTR
    Tc-U6B-Uni
    SV40polyA
    Tc-vermilion
    3xP3 TATA
    pBacR
    

    Expression

    Insect

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBac(3xP3-gTc’v; Valcyrie.LR)

    Plasmid

    #119066

    Purpose

    Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2019 Jan 4;146(1). pii: dev

    Inserts

    pBacL
    Tc-U6a-Lox.L
    Tc-hsp68 Promoter
    NLS-Cas9
    Tc-hsp68 3'UTR
    Tc-U6B-Lox.R
    SV40polyA
    Tc-vermilion
    3xP3 TATA
    pBacR
    

    Expression

    Insect

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIDS-LSD1-C

    Plasmid

    #109160

    Purpose

    Encodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACEBAC1-CoREST-C

    Plasmid

    #109159

    Purpose

    Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOCC17

    Plasmid

    #118875

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOCC16

    Plasmid

    #118870

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    

    Tags

    EGFP and HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOCC218

    Plasmid

    #118907

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS10, cleavable with 3C protease, YBBR tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOCC18

    Plasmid

    #118868

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOCC111

    Plasmid

    #118906

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
    

    Tags

    LPETGg, HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOCC176

    Plasmid

    #118891

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOCC171

    Plasmid

    #118888

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pOCC202

    Plasmid

    #118880

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    mKate2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOCC139

    Plasmid

    #118872

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC 3GLA

    Plasmid

    #71762

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSGE_886_Nhe2_EcR-to-ato_RB

    Plasmid

    #71181

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    886_Nhe2_EcR-to-ato_RB
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    DSCP Drosophila synthetic core promoter

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFL I3

    Plasmid

    #113514

    Purpose

    Expression of I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFL His-I3 V41A / W43A

    Plasmid

    #113515

    Purpose

    Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His

    Expression

    Insect

    Mutation

    V41A /W43A

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge