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pKM634

Plasmid

#134219

Purpose

Baculovirus expression of CENP-HIKM

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP HIKM

Expression

Insect

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pDK580

Plasmid

#134205

Purpose

Baculovirus expresion of CENP-L and CENP-N

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-L and His-CENP-N

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM412

Plasmid

#134213

Purpose

Baculovirus expression of CENP-P and CENP-O

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP-P + O

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM672

Plasmid

#134221

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 1-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Myo 9 Avi+Flag

Plasmid

#134969

Purpose

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

Depositor

Martin Bähler

Article

Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

Insert

Myo 9 (hum-7 Nematode)

Tags

Avi and Flag

Expression

Insect

Promoter

Polyhedrin

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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U6d-T

Plasmid

#117212

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

u6d promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFast-dCry-V5His

Plasmid

#136240

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Insect

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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DYNLL2

Plasmid

#132531

Purpose

Human LC8-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL2 (DYNLL2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLT3

Plasmid

#132532

Purpose

Human TCTEX-3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT3 (DYNLT3 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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LC8-like

Plasmid

#133115

Purpose

Human LC8-like protein, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

LC8-like (LOC392067 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67B human Fzd4 CRD (38–160)

Plasmid

#132621

Purpose

expresses human Fzd4 CRD (38-160) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

Insert

Fzd4 CRD (38–160) (FZD4 Human)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pCG6770 eGFP SV40

Plasmid

#132649

Purpose

Expresses eGFP mRNA from the Drosophila CG6770 promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

CG6770

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_ppst eGFP SV40

Plasmid

#132648

Purpose

Expresses eGFP mRNA from the Drosophila pst promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

pst

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pSirup eGFP SV40

Plasmid

#132647

Purpose

Expresses eGFP mRNA from the Drosophila Sirup promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Sirup

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge