5,013 results
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Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBDP
Plasmid#17566DepositorInsertmini w+
ExpressionInsectAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKLBac-FluB-trimer
Plasmid#229859PurposeInfluenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)DepositorInsertsPA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)
Tags2x-Streptag and 8xHis-tagExpressionInsectAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3xVHH05-DsRed
Plasmid#171581PurposeFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC153
Plasmid#118901Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
TagsTwinStrep, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pattB-DSCP-QF#7-hsp70
Plasmid#46133DepositorInsertQF#7
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
ExpressionInsectAvailable SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20XUAS-IVS-PhiC31
Plasmid#133565PurposeReplace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRExpressionInsectPromoterUASTAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6L
Plasmid#198409PurposeExpresses FLAG-tagged human IntS6LDepositorInsertIntegrator complex subunit 6 like (INTS6L Human)
Tags3x FLAGExpressionInsectPromoterUbi-p63eAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
NHR1+2-Flag pBacPAK8-3
Plasmid#17324DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.SUZ12
Plasmid#125172PurposeExpresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4
Plasmid#217893PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)DepositorExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-CR7T-gRNA2.1-nlsBFP
Plasmid#170515PurposegRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterCR7TAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRExpressionInsectAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-HA-STX1
Plasmid#89310PurposeExpresses HA tagged syntaxin 1 in Drosophila cellsDepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKSB-618 U6-sgRNA2/3 GGAA AACA
Plasmid#173674PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_HCK
Plasmid#224245PurposeExpression of human HCK Kinase in insect cells (SF9 cells)DepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rabx6 WT
Plasmid#53479DepositorAvailable SinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-I539E
Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
AlphaC
Plasmid#124525PurposepColE1_AlphaCDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE3c
Plasmid#17463Purposemodified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#214139PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
TagsGST and His-MBPExpressionInsectMutationnoneAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT-Diaphanous-GFP
Plasmid#24287DepositorAvailable SinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only