4,932 results

1. pCoofy63

   Plasmid

   #122015

   Purpose

   Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-Smt3Star and Multi3: depending on the LP2 pr…

   Expression

   Insect

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJFRC170-3XUAS-IVS-Cre::PEST

   Plasmid

   #32144

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   Cre::PEST
   

   Expression

   Insect

   Available Since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-mCherry-LINXb3

   Plasmid

   #73615

   Purpose

   Light-induced nuclear export switch for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXb3
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC175

   Plasmid

   #118890

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASTattB-p65AD::CaM

   Plasmid

   #64717

   Purpose

   generating transgenic TRIC flies

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   p65AD::CaM
   

   Expression

   Insect

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. UAS-Syt::3xHalo7

   Plasmid

   #87649

   Purpose

   For creating drosophila transgenics expressing UAS-Syt::3xHalo7

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Syt::3xHalo7
   

   Expression

   Insect

   Mutation

   S265G in Syt (please see depositor's commen…

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACEBac1-beta-actin

   Plasmid

   #178076

   Purpose

   Insect cell expression of beta-actin

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   beta-actin (ACTB Human)
   

   Expression

   Insect

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Plasmid

   #159577

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Insect

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

    Plasmid

    #159578

    Purpose

    Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGP67A human LRP6 E1E2 (20–629)

    Plasmid

    #132627

    Purpose

    expresses human LRP6 E1E2 (20–629) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–629)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCaSper4 cyto roGFP2-Orp1

    Plasmid

    #64998

    Purpose

    Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSTEP-SNAPf

    Plasmid

    #72335

    Purpose

    Tissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)

    Depositor

    Avital Rodal

    Article

    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

    Insert

    RRS-TagRFPT-p10-[Golic+] RRS-SNAPf
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xTRE DREaMR 3p3GFP

    Plasmid

    #182205

    Purpose

    3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    rTetR-3XP3-GFP
    

    Expression

    Insect

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. DmU6 3p3 GFP LgRNA

    Plasmid

    #182207

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3XP3-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EKAR2G_design1_mTFP_105_Venus_157

    Plasmid

    #39819

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pS3aG

    Plasmid

    #31171

    Purpose

    A Drosophila GFP reporter transgene vector for site-specific integration

    Depositor

    Thomas Williams

    Article

    Williams et al Cell. 2008 Aug 22;134(4):610-23. doi: 10

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pJC-TALE-GFP

    Plasmid

    #46148

    Depositor

    David Stern

    Article

    Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. 20XUAS IVS Jaws mCherry_tr

    Plasmid

    #111549

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Biao Wang, Marc Montminy

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pMT Draper-mCherry

    Plasmid

    #126667

    Purpose

    Inducible expression of Draper-mCherry in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper mCherry
    

    Expression

    Insect

    Mutation

    C813Y- see depositor comments below

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABCB8 (ABCB8A-c016)

    Plasmid

    #110266

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ABCB8 (ABCB8 Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac mph1 flag

    Plasmid

    #1985

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    mPh1 (Phc1 Mouse)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pOPINO

    Plasmid

    #41140

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

    Plasmid

    #126024

    Purpose

    express RFP-tagged LARIAT for optogenetic clustering

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CIBN-MP; CRY2 PHR-VHH(GFP)
    

    Tags

    RFP

    Expression

    Insect

    Mutation

    WT

    Promoter

    Hsp70

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

    Plasmid

    #63170

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-OLLAS"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. OA-1055J

    Plasmid

    #200251

    Purpose

    pBac-U6-gRNA(Intersex)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    2 gRNAs targeting Intersex gene
    

    Expression

    Insect

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPTQF#7-hsp70

    Plasmid

    #46136

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUAS myc Gr66a

    Plasmid

    #26861

    Depositor

    Craig Montell

    Article

    Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

    Insert

    Gr66 gustatory receptor (Gr66a Fly)
    

    Tags

    myc

    Expression

    Insect

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pUAST-SIK3 WT

    Plasmid

    #52972

    Purpose

    insect expression of SIK3 WT

    Depositor

    Biao Wang, Marc Montminy

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

32. p2X-UASTattB

    Plasmid

    #189860

    Purpose

    Fly cloning and transformation

    Depositor

    Jung Hwan Kim

    Article

    Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. psiCHECK2-IRES-let-7 8x

    Plasmid

    #20932

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

    Insert

    8x let-7 target sites
    

    Use

    Luciferase

    Expression

    Insect and Mammalian

    Mutation

    168bp IRES from 5'UTR of reaper gene in Dros…

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pJFRC173-20XUAS-IVS-Dre::PEST

    Plasmid

    #32146

    Purpose

    Expresses Dre recombinase under control of 20XUAS sites in Drosophila

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Dre::PEST
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EKAR2G_design1_mTFP_105_Venus_195

    Plasmid

    #39821

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

36. EKAR2G_design1_mTFP_159_Venus_173

    Plasmid

    #39825

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EKAR2G_design1_mTFP_175_Venus_195

    Plasmid

    #39831

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

38. U6b-T

    Plasmid

    #117210

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    u6b promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMKInt

    Plasmid

    #18952

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Expression

    Insect

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

40. EKAR2G_design1_mTFP_wt_Venus_wt

    Plasmid

    #39813

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge