-
Plasmid#135620PurposeExpression of SpyTag-B2AR in insect cellDepositorInsertBeta 2 adrenergic receptor (ADRB2 Human)
UseTagsHA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tagExpressionInsectMutationDeleted 1-24 aa, 366-413 aaPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
T6-pAC5.1 mEGFP-EcoRV
Plasmid#206489PurposeSchneider cell imagingDepositorTypeEmpty backboneUseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOCC114
Plasmid#118904Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase siteDepositorInsertNcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with TEV protease, two glycinesExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)
Plasmid#55223DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
OA-1067K
Plasmid#164848PurposeExpresses four gRNA targeting Mhc gene with 3xp3-tdTomato markerDepositorInsert4 gRNAs and 3xP3-tdTomato
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-GAL4QF
Plasmid#61309PurposeExpresses chimeric transactivator GAL4_DBD:QF2w_AD in DrosophilaDepositorInsertGAL4_DBD::QF2w_AD
UseTagsExpressionInsectMutationChanged EK on C-terminus of QF2 to KKKKPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL4DBD
Plasmid#26256DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
OA-874PA
Plasmid#164846PurposeExpresses AAEL005635-Cas9 with OpIE2-CFP markerDepositorInsertP10-3'UTR OpIE2-CFP
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-RpS12
Plasmid#71504PurposeflySTARR-seq screening vector with RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBXC3GH
Plasmid#49051PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectMutationPromotermetallothioneinAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE1c
Plasmid#17461Purposemodified pENTR vector for C-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAC-QSco
Plasmid#46106DepositorInsertQSco
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Shp2)
Plasmid#177899PurposeBaculo expression of His10 tag fused to human Shp2DepositorInsertShp2 (PTPN11 Human)
UseTags10xHis-TEV-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterActin5CAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
K20‐scFv‐pSMBP2
Plasmid#159940PurposeTo generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20DepositorInsertanti beta-1 integrin scFv
UseTags6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…ExpressionInsectMutationPromoterPolyhedrinAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EZH2
Plasmid#125171PurposeExpresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertEZH2 (EZH2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
UseTagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBFv-U6.2B
Plasmid#138401PurposegRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vectorDepositorInsertU6-2
UseTagsExpressionInsectMutationPromoterU6-2Available sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 WT
Plasmid#44824DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Nucleocapsid
Plasmid#179696PurposeExpresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cellsDepositorInsertSARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
UseTagsHis6-TEV protease siteExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KCNK2
Plasmid#73257PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertKCNK2 (KCNK2 Human)
UseTagsTEV-His10-FLAGExpressionInsectMutationuserdefined:Full_Length 1411PromoterPolyhedrinAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFHMSP-LIC-N
Plasmid#26095PurposeDonor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expressionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis and TEV cleavage siteExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 15, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcRP67A prM/E YFV
Plasmid#52883Purposeexpression of Yellow Fever Virus VLPs in baculovirusDepositorInsertprM/E
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Grx1
Plasmid#65000PurposeExpression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
Plasmid#133560PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
OA-961B
Plasmid#104967PurposeD. suzukii Medea ConstructDepositorInsertsGFP
dsRed
HR5Ie1
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only