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Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF2
Plasmid#185443PurposeExpresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF2 (TERF2 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
RIPK2 (RIPK2A-c036)
Plasmid#110268PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRIPK2 (RIPK2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AT5G48740 O3_pECIA2
Plasmid#114963PurposeBait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.DepositorInsertAT5G48740 (AT5G48740 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT5G59650 O4_pECIA2
Plasmid#114964PurposeBait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.DepositorInsertAT5G59650 (AT5G59650 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC159-1XUAS-DSCP-B3::PEST
Plasmid#32137DepositorInsertB3::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
p13xLexAOP2-IVS-Syn21-Voltron-p10
Plasmid#119042PurposeLexAop2-driven expression of Voltron in DrosophilaDepositorInsertVoltron
UseTagsExpressionInsectMutationPromoterhsp70Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCoofy59
Plasmid#122011PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage siteDepositorTypeEmpty backboneUseTagsTrxA-His6ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pfast NPC1(NTD)
Plasmid#25773DepositorInsertnpc1 (NPC1 Human)
UseTagsHisExpressionInsectMutationaa 23-252 onlyPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSGE_768_Nhe2-EcR_dCP
Plasmid#71223PurposeLuciferase reporterDepositorInsert768_Nhe2-EcR_dCP
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGE_769_DipB-EcR_dCP
Plasmid#71222PurposeLuciferase reporterDepositorInsert769_DipB-EcR_dCP
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFS337-USP44-FLAG
Plasmid#43905DepositorInsertUSP44 (USP44 Human)
UseBaculoviral expressionTagsFLAGExpressionInsectMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-SNAPf-Psc-StrepII
Plasmid#111860PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.DepositorInsertHook3(1-552)-SNAPf (HOOK3 Human)
UseTagsPsc, SNAPf, and Strep-IIExpressionInsectMutationOptimised for expression in Sf9 cellsPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_195
Plasmid#40002DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXN-FBLWLF-GAL4DBD
Plasmid#26264DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169164PurposeBaculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cellsDepositorInsertnsp14/nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
UseTags6His-3xFlag (nsp10 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)
Plasmid#169166PurposeBaculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cellsDepositorInsert3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_J
Plasmid#146709PurposeInsect Expression of DmNot1DepositorInsertDmNot1 (Not1 Fly)
UseTagsExpressionInsectMutationThree non silent mutations N605S, K759M, G1999S a…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
Plasmid#71812PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSK24
Plasmid#72852Purposetemplate for C-terminal Twin-Strep tag, Puromycin-resistanceDepositorInserttwin Strep tag
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGRY
Plasmid#61599PurposeTransformation vector pBGRYDepositorTypeEmpty backboneUseDrosophila transformation vectorTagsExpressionInsectMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only