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pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Tags

6x HIS

Expression

Insect

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB087

Plasmid

#228479

Purpose

"Empty" pLib vector (biGBac System) for easy DNA Assembly

Depositor

Richard Baker

Type

Empty backbone

Expression

Insect

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L786A

Plasmid

#228838

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L786A

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pM.004

Plasmid

#228690

Purpose

C terminal dual protein A affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

TEV-2xProtein A

Expression

Insect

Mutation

WT

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pM.003

Plasmid

#228689

Purpose

N terminal dual protein A affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

2xProtein A-TEV

Expression

Insect

Mutation

WT

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)

Plasmid

#228833

Purpose

Insect cell expression of CC3 domain of BICD2 (aa 711-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pM.010

Plasmid

#228696

Purpose

C terminal Strep affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

TEV-Strep

Expression

Insect

Mutation

WT

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pM.009

Plasmid

#228695

Purpose

N terminal Strep affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Strep-TEV

Expression

Insect

Mutation

WT

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pM.008

Plasmid

#228694

Purpose

N terminal 3xFlag-6xHis affinity tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

3xFlag-6xHis

Expression

Insect

Mutation

WT

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1e

Plasmid

#228679

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1d

Plasmid

#228678

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1c

Plasmid

#228677

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB.BIG1b

Plasmid

#228676

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1a

Plasmid

#228675

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.008

Plasmid

#228674

Purpose

tSV40 Terminator for Baculovirus expression with Cterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

tSV40

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.007

Plasmid

#228673

Purpose

tSV40 Terminator for Baculovirus expression without Cterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

tSV40

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.006

Plasmid

#228672

Purpose

polH Promoter for Baculovirus expression with Nterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

polH

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.005

Plasmid

#228671

Purpose

polH Promoter for Baculovirus expression without Nterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

polH

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.004

Plasmid

#228670

Purpose

Recombination to BV DNA + SpecR (Tn7, SpecR)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Tn7L, SpecR

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB.003

Plasmid

#228669

Purpose

Recombination to BV DNA (Tn7)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Tn7L

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB.002

Plasmid

#228668

Purpose

Moclo Baculo toolkit level 2 backbone

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB.001

Plasmid

#228667

Purpose

Moclo Baculo toolkit level 1 backbone

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM.002

Plasmid

#228688

Purpose

C terminal MBP affinity tag with PreScission Protease cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Prs-MBP

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM.001

Plasmid

#228687

Purpose

N terminal MBP affinity tag with PreScission Protease cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

MBP-Prs

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(1-400)

Plasmid

#228830

Purpose

Insect cell expression of N-terminal portion of BICD2 (aa 1-400)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 1-400

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1_Strep-Psc-BICD2

Plasmid

#228829

Purpose

Insect cell expression of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1_Strep-Psc-BICD2(342-800)

Plasmid

#228832

Purpose

Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 342-800

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1_Strep-Psc-BICD2(711-800)_L742A

Plasmid

#228839

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L742A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac SOX 318-320A

Plasmid

#192060

Purpose

Expression vector for SOX (ORF37) 318-320A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Expression

Insect

Mutation

318-320A

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Article

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

Plasmid

#222300

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[451-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

Plasmid

#222301

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[571-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacHTA-NEDD4L_FL

Plasmid

#187722

Purpose

Express His-tagged NEDD4L FL in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

Plasmid

#187723

Purpose

Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 1-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacHTA-NEDD4L_W1-L-W2-HECT

Plasmid

#187724

Purpose

Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 153-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 1-152 and 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dm_λHA-FLuc100_CSC

Plasmid

#210592

Purpose

Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dm_λHA-FLuc0_CSC

Plasmid

#210589

Purpose

Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)

Use

Luciferase

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacI-KDAC7 CD

Plasmid

#224345

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSN539

Plasmid

#226775

Purpose

Expresses Drosophila UNC-104(wild-type) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC51

Plasmid

#226779

Purpose

Expresses Drosophila UNC-104(A255V) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (A255V) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Alanine 255 to Valine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

mEGFP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Tags

HMBP

Expression

Insect

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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