4,908 results

1. pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

   Plasmid

   #32143

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   RSRT> STOP STOP RSRT>
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOEM-GED-EGFP

   Plasmid

   #102782

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, eGFP

   Depositor

   Elly Tanaka

   Article

   Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

   Insert

   EGFP
   

   Use

   Baculoviral rescue shuttle vector

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExpreS2-1-C3-PA

   Plasmid

   #175446

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   3C-Protein A and BiP Secretion Peptide

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. RBBP4

   Plasmid

   #25350

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RBBP4-13 (RBBP4 Human)
   

   Use

   Baculovirus expression

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pLib6.6

   Plasmid

   #176652

   Purpose

   Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMFB2

   Plasmid

   #31278

   Depositor

   Aziz Sancar

   Article

   Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

   Insert

   Bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pUAST-SIK3 T196A

   Plasmid

   #52974

   Purpose

   insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

   Depositor

   Biao Wang, Marc Montminy

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   T196A LKB1 phosphorylation defective mutant

   Promoter

   Hsp70

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pUAST-SIK3 K70M

   Plasmid

   #52973

   Purpose

   insect expression of SIK3 K70M (kinase dead mutant)

   Depositor

   Biao Wang, Marc Montminy

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   K70M kinase dead mutant

   Promoter

   Hsp70

   Available since

   May 5, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pKM394

    Plasmid

    #134211

    Purpose

    Baculovirus expression of CENP-N and CENP-L

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-N-his-CENP-L
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-OSF-TRIM5-21R

    Plasmid

    #79063

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5-21R
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pattB-DSCP-QF-sv40

    Plasmid

    #24369

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QF
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK2-let-7 2x

    Plasmid

    #20929

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

    Insert

    2x let-7 target sites
    

    Use

    Luciferase

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pJFRC158-3XUAS-IVS-B3::PEST

    Plasmid

    #32138

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    B3::PEST
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCasper4-QF#7-hsp70

    Plasmid

    #46135

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1154B_gFLE_pB_VTK_ActG

    Plasmid

    #187238

    Purpose

    An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

    Depositor

    Omar Akbari

    Article

    Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

    Insert

    Femaleless
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMKInt-hyg

    Plasmid

    #18953

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pRB34

    Plasmid

    #72862

    Purpose

    template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    3xFlag tag
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159672

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    βTub-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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