4,908 results

1. pPVxRF3

   Plasmid

   #138385

   Purpose

   Venus knock-in cassette

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   Venus
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ths pUAST-HA

   Plasmid

   #69769

   Purpose

   Thisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

   Depositor

   Arno Muller

   Article

   Klingseisen et al Development. 2009 Jul;136(14):2393-402.

   Insert

   Thisbe (FGF8like-1)
   

   Use

   P element-based puast vector for gal4-regulated e…

   Tags

   HA-Tag

   Expression

   Insect

   Mutation

   Encodes amino acids 1-739 of Thisbe (NP_610701.2)…

   Promoter

   hsp70 promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Pyr pUAST-HA

   Plasmid

   #69768

   Purpose

   Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

   Depositor

   Arno Muller

   Article

   Klingseisen et al Development. 2009 Jul;136(14):2393-402.

   Insert

   Pyramus (FGF8like-2) (pyr Fly)
   

   Use

   P element-based puast vector for gal4-regulated e…

   Tags

   HA-Tag

   Expression

   Insect

   Mutation

   241T amino acid insertion compared to reference s…

   Promoter

   hsp70 promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pKM410

   Plasmid

   #134212

   Purpose

   Baculovirus expression of CENP-T and CENP-W

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   GST-CENP-T+W
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASTP2

   Plasmid

   #13843

   Depositor

   Ting Wu

   Article

   Bateman et al Genetics. 2006 Jun . 173(2):769-77.

   Insert

   mini-white (w Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac-CHD7 (1-1899)

   Plasmid

   #170143

   Purpose

   Expresses residues 1-1899 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal truncation of residues 1900-2997

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-CHD7 (787-1899)

   Plasmid

   #170145

   Purpose

   Expresses residues 787-1899 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   N-terminal truncation of residues 1-786; C-termin…

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-CHD7 (787-2138)

   Plasmid

   #170146

   Purpose

   Expresses residues 787-2138 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   N-terminal truncation of residues 1-786; C-termin…

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac-CHD7 (1-1547)

   Plasmid

   #170144

   Purpose

   Expresses residues 1-1547 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal truncation of residues 1548-2997

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-CHD7 (936-2997)

    Plasmid

    #170147

    Purpose

    Expresses residues 936-2997 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    N-terminal truncation of residues 1-935

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac-CHD7 (K999R)

    Plasmid

    #170142

    Purpose

    Expresses CHD7 (K999R) in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Insect

    Mutation

    K999R

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p10xUAS-IVS- Syn21-Voltron-p10

    Plasmid

    #119041

    Purpose

    UAS-driven expresion of Voltron in Drosophila

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. rNL1(46-638)

    Plasmid

    #20172

    Depositor

    Axel Brunger

    Article

    Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

    Insert

    Neuroligin 1 (Nlgn1 Rat)
    

    Use

    Tags

    C-terminal 6X His tag

    Expression

    Insect

    Mutation

    no splice sites

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

14. 57 pLIB_C_CBP

    Plasmid

    #160306

    Purpose

    C-term uncleavable CBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDSAY

    Plasmid

    #62291

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMos-3xP3DsRed-attP

    Plasmid

    #52904

    Purpose

    Mos1 transformation vector containing MCS, loxP, attP, 2 x 5HE

    Depositor

    Zach Adelman

    Article

    Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPRR2A-pFastBac I

    Plasmid

    #182460

    Purpose

    Expression His-tagged SPRR2A in insect cells

    Depositor

    Lora Hooper

    Article

    Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

    Insert

    small proline rich protein 2A (SPRR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHD-mW-attPCC-FRT (pTL717)

    Plasmid

    #115158

    Purpose

    Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    mW attP-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge