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5,017 results

Showing: 1301 - 1320 of 5017 results

  3. pAc5.1B-MS2_V

    Plasmid
    #147737
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    147737-plasmid-map-sequence-id-357450

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  4. Fast-GCaMP-EF05

    Plasmid
    #52558
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF05
    Expression
    Insect
    Mutation
    changed from GCaMP3: D397N/G398A/N399D
    Available Since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pOCC114

    Plasmid
    #118904
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEV protease, two glycines
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118904-plasmid-map-sequence-id-232331

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  13. pTSTEP-SNAPf

    Plasmid
    #72335
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)
    Depositor
    Avital Rodal
    Article
    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.
    Insert
    RRS-TagRFPT-p10-[Golic+] RRS-SNAPf
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72335-plasmid-map-sequence-id-132005

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  15. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

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  19. Or1a-Gal4

    Plasmid
    #63019
    Purpose
    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4
    Depositor
    Leslie Vosshall
    Article
    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.
    Insert
    Drosophila melanogaster Or1a promoter
    Tags
    Drosophila ADH (aa's 1-18) fused to Yeast Ga…
    Expression
    Insect
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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Showing: 1301 - 1320 of 5017 results