4,908 results

1. pUASP dsRed Rab11 WT

   Plasmid

   #44829

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab11 (Rab11 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pExpreS2-1-CR-PA-10H-SIII

   Plasmid

   #175447

   Purpose

   HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   BiP Secretion Peptide and Thrombin-Protein A-10Hi…

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BICD2N

   Plasmid

   #64070

   Purpose

   N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   BICD2N (1-400) (Bicd2 Mouse)
   

   Use

   Tags

   GFP and His-ZZ-TCS

   Expression

   Insect

   Mutation

   Codon optimized for Sf9 expression

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLib6.4B

   Plasmid

   #176665

   Purpose

   Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nup98-pDONR221

   Plasmid

   #124241

   Purpose

   Gateway entry clone of Drosophila Nup98-96 cDNA.

   Depositor

   Peter Vilmos

   Article

   Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

   Insert

   Drosophila Nup98-96 (Nup98-96 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2Bac.V5.p62

   Plasmid

   #39218

   Depositor

   Aziz Sancar

   Insert

   p62 (GTF2H1 Human)
   

   Use

   Tags

   V5

   Expression

   Insect

   Mutation

   Promoter

   Pp10

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCaSpeR-act5cB-QF#7m1

   Plasmid

   #46126

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7m1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-7-QFBDAD

   Plasmid

   #46096

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSBL_DK193

   Plasmid

   #156424

   Purpose

   Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (Q57H)
   

   Use

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   Q57H

   Promoter

   Polyhedrin

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ninaE-dsocs

    Plasmid

    #26857

    Depositor

    Craig Montell

    Article

    Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

    Insert

    Slow Termination of Phototransduction (stops Fly)
    

    Use

    Tags

    myc

    Expression

    Insect

    Mutation

    This STOPS construct contains a mutated/truncated…

    Promoter

    Available since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pACUH-GFP1-10 mitochondrial matrix

    Plasmid

    #172063

    Purpose

    UAS vector to express GFP1-10 in the mitochondrial matrix

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP1-10 mitochondrial matrix
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBacPAK8-His-myc-Rbx1

    Plasmid

    #29506

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Rbx1 (Rbx1 Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pFastBac K-M33

    Plasmid

    #1986

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

14. 10XUAS IVS Jaws mVenus_tr

    Plasmid

    #111553

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mVenus_tr
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PRE-Hsp70BbCas9_1.3

    Plasmid

    #190797

    Purpose

    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASTattb-cAMPr Y205A, R739A

    Plasmid

    #84933

    Purpose

    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr Y205A R739A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    PKA-C Y205A, PKA-R R739A

    Promoter

    5X UAS

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAct5C-Hh

    Plasmid

    #37382

    Depositor

    Norbert Perrimon

    Article

    Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

    Insert

    hedgehog (hh Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    actin5C

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

18. p2Bac.Flag.p34

    Plasmid

    #39221

    Depositor

    Aziz Sancar

    Insert

    p34 (GTF2H3 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Pph

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-DSCP-Gateway

    Plasmid

    #71507

    Purpose

    flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUASTattb-cAMPr Y205A, R626A

    Plasmid

    #84932

    Purpose

    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr Y205A R626A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    PKA-C Y205A, PKA-R R626A

    Promoter

    5X UAS

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge