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pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

Plasmid

#32147

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

rox> STOP STOP rox>

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pOCC193

Plasmid

#118903

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette

Tags

MBP, cleavable with 3C protease; mGFP and TwinStr…

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Tags

eGFP

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJC-TALE-VP64

Plasmid

#46147

Depositor

David Stern

Article

Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

Type

Empty backbone

Tags

VP64 activation domain

Expression

Insect

Promoter

hsp70

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSBL_DK193

Plasmid

#156424

Purpose

Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (Q57H)

Tags

sfGFP-His

Expression

Insect

Mutation

Q57H

Promoter

Polyhedrin

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G35710 X075_pECIA2

Plasmid

#115098

Purpose

Bait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G35710 (AT1G35710 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G56140 X032_pECIA2

Plasmid

#115062

Purpose

Bait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56140 (AT1G56140 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G06840 X062_pECIA2

Plasmid

#115052

Purpose

Bait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G06840 (AT1G06840 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac FPC

Plasmid

#1927

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

Polycomb (Pc Fly)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
AT2G19230 M11_pECIA2

Plasmid

#114949

Purpose

Bait vector AT2G19230 M11_pECIA2 should be used with prey vector AT2G19230 M11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G19230 (AT2G19230 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G29730 X066_pECIA14

Plasmid

#114855

Purpose

Prey vector AT1G29730 X066_pECIA14 should be used with bait vector AT1G29730 X066_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G29730 (AT1G29730 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT3G02880 J3_pECIA14

Plasmid

#114790

Purpose

Prey vector AT3G02880 J3_pECIA14 should be used with bait vector AT3G02880 J3_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G02880 (AT3G02880 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G51890 M5_pECIA14

Plasmid

#114744

Purpose

Prey vector AT1G51890 M5_pECIA14 should be used with bait vector AT1G51890 M5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G51890 (AT1G51890 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pXN-attPGal4LWL

Plasmid

#26261

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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HS-H3.3A-GFP

Plasmid

#8696

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A (His3.3A Fly)

Tags

GFP

Expression

Insect

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pKM410

Plasmid

#134212

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-T+W

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDSAY-vasaCas9sv40

Plasmid

#173669

Purpose

Cas9 expression vector for Anopheles

Depositor

Eric Marois

Insert

Cas9 under control of vasa promoter and SV40 terminator

Use

CRISPR

Expression

Insect

Promoter

vasa

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPromoterless-P2A

Plasmid

#165790

Purpose

Viral P2A part in promoter grammar, for building promoterless control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless-2A

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Orp1

Plasmid

#64997

Purpose

for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac K-M33

Plasmid

#1986

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Expression

Insect

Mutation

Kozak sequence added before ATG

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only