4,908 results

1. pRB36

   Plasmid

   #72861

   Purpose

   template base vector for N-terminal tagging, constitutive Blasticidin resistance

   Depositor

   Klaus Foerstemann

   Article

   Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

   Insert

   base vector for introduction of tags
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-WingGFP-tan

   Plasmid

   #83473

   Purpose

   EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   WingGFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Hsp70 and enhancer from D. melanogaster yellow ge…

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGB-08-09-BECN1

   Plasmid

   #187991

   Purpose

   Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325

   Depositor

   Sascha Martens

   Insert

   BECN1 (BECN1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAST-HA-dAtg13

   Plasmid

   #111802

   Purpose

   Insect Expression of dAtg13

   Depositor

   Jun Hee Lee

   Article

   Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

   Insert

   dAtg13 (Atg13 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

   Plasmid

   #146503

   Purpose

   Insect Expression of DmDCP1_327-372

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   DmDCP1_327-372 (Dcp1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINH

   Plasmid

   #53537

   Purpose

   pTriex-based vector with signal sequence and N-his tag for protein expression

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac FPSC

   Plasmid

   #1926

   Depositor

   Robert Kingston

   Article

   Francis et al Mol Cell 2001 Sep;8(3):545-56.

   Insert

   posterior sex combs (Psc Fly)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pUAST-attB-Flag-dTSC2 WT

   Plasmid

   #111807

   Purpose

   Insect Expression of dTSC2

   Depositor

   Jun Hee Lee

   Article

   Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

   Insert

   dTSC2 (gig Fly)
   

   Use

   Tags

   3xFlag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBP-p65AD::CaM

   Plasmid

   #64719

   Purpose

   generating transgenic TRIC flies

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   p65AD::CaM
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBacPAK8-ElonginC

    Plasmid

    #29505

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Elongin C (ELOC Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pFastBac K-Ring1A

    Plasmid

    #1994

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    Ring1A (Ring1 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Kozak before start codon

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pAc-His-zfCsf1a

    Plasmid

    #168103

    Purpose

    Express soluble His-tagged zebrafish Csf1a in insect cells

    Depositor

    Petr Bartunek

    Article

    Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

    Insert

    Csf1a (csf1a Zebrafish)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASP YFP Rab4 DN

    Plasmid

    #37691

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab4 (Rab4 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S22N (dominant negative)

    Promoter

    GAL4

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. ArpC5_pLib

    Plasmid

    #173682

    Purpose

    ArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC5 (ARPC5 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ArpC3-FLAG_pLib

    Plasmid

    #173680

    Purpose

    ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC3 (ARPC3 Human)
    

    Use

    Tags

    TEV cleavage site and FLAG tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ArpC1_pLib

    Plasmid

    #173678

    Purpose

    ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC1 (ARPC1B Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRIV-Gal4DBD:2xnMagHigh1

    Plasmid

    #182031

    Purpose

    Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

    Depositor

    Jean-Paul Vincent

    Article

    di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

    Insert

    Gal4DBD2xHighMag1-p103'UTR
    

    Use

    Donor plasmid

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 892 pFastBacHTb PTEN

    Plasmid

    #10938

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ORCO-T2A-QF2

    Plasmid

    #162524

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge