4,958 results
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Plasmid#186144PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
TagsHis6ExpressionInsectMutationDNA mutation T796G;G797C Amino acid mutation W266APromoterpolyhedrin promoterAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lck
Plasmid#186130PurposeFor expression of recombinant Lck in SF9 cells.DepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK Pyk2
Plasmid#186146PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
TagsHis6ExpressionInsectMutationC-terminal linker swapped with Pyk2 linkerPromoterpolyhedrin promoterAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
BMP10 N67Q N131Q D4G (S2-2)
Plasmid#184622Purposefor transfection into S2 cells and expression of BMP10 proteinDepositorInsertBMP10 (BMP10 Human)
Tags8x His - streptavidin binding peptideExpressionInsectMutationN67Q, N131Q, ARIRR(312-316) replaced with GAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056H
Plasmid#183135PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056J
Plasmid#183136PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056K
Plasmid#183137PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_340-368-GST-V5His6_AH
Plasmid#148935PurposeInsect Expression of DmGIGYF_340-368DepositorInsertDmGIGYF_340-368 (Gyf Fly)
ExpressionInsectAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN526
Plasmid#184827PurposeExpresses human KIF5A(∆exon27) in insect cellsDepositorInsertKIF5A (∆exon27) (KIF5A Human)
TagsmScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterpolyhedrinAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-A253FY208A_X
Plasmid#147973PurposeInsect Expression of DmCaf40-A253FY208ADepositorAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z
Plasmid#148160PurposeInsect Expression of Dm4E-T_1-511-LLFWKtoAAAAADepositorInsertDm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
ExpressionInsectMutationalias: 4E-T; one non silent mutation L127S, annot…Available SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6C_L
Plasmid#146855PurposeInsect Expression of HsTNRC6CDepositorInsertHsTNRC6C (TNRC6C Human)
ExpressionInsectMutationone non silent mutation Q1183K compard to the seq…Available SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppBAalbNixE1E3E4 PUb-YFP
Plasmid#173666Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
YFP
TagsSV40 NLSExpressionInsectMutationNix isoforms with exons E1, E3, E4PromoterAedes aegypti PUb and ownAvailable SinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6A_L
Plasmid#146843PurposeInsect Expression of HsTNRC6ADepositorInsertHsTNRC6A (TNRC6A Human)
ExpressionInsectMutationA339T, K1578E compared to the sequence of isoform…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T
Plasmid#147580PurposeInsect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666DepositorInsertDm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)
ExpressionInsectAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-FlucV5His6-PolyA-HhR_AB
Plasmid#148303PurposeInsect Expression of PolyA-HhRDepositorInsertPolyA-HhR
ExpressionInsectAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q
Plasmid#147340PurposeInsect Expression of DmNot4-dsRNAresDepositorInsertDmNot4-dsRNAres (CG31716 Fly)
ExpressionInsectMutation7 silent mutations and 6 non silent mutations G20…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-L36AL39A_T
Plasmid#147604PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
ExpressionInsectMutationTwo non silent mutations (H628R and N659S) compar…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q
Plasmid#147279PurposeInsect Expression of DmNot3-dsRNAresDepositorInsertDmNot3-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q
Plasmid#147274PurposeInsect Expression of DmeIF4Ga-Y80A-dsRNAresDepositorInsertDmeIF4Ga-Y80A-dsRNAres (eIF4G1 Fly)
ExpressionInsectMutationone non silent mutation K340R compared to the seq…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q
Plasmid#147262PurposeInsect Expression of DmNot3-F818A-dsRNAresDepositorInsertDmNot3-F818A-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-10xmut_G
Plasmid#146428PurposeInsect Expression of DmGW182-10xmutDepositorInsertDmGW182-10xmut (gw Fly)
ExpressionInsectMutationMultiple mutations compared to NM_166780.1, see a…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P
Plasmid#147209PurposeInsect Expression of DmXRN1-P1339AP1342A-dsRNAresDepositorInsertDmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J
Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
ExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
ExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R
Plasmid#147394PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389ADepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Human, Nematode)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O
Plasmid#147065PurposeInsect Expression of DmXRN1-D207A-dsRNAresDepositorInsertDmXRN1-D207A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N
Plasmid#146969PurposeInsect Expression of DmXRN1-del1323-1355-dsRNAresDepositorInsertDmXRN1-del1323-1355-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delCterm_E
Plasmid#146252PurposeInsect Expression of DmGW182-delC-termDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E
Plasmid#146241PurposeInsect Expression of DmSMG6-siRNAresDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
TagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔN
Plasmid#182840PurposeA 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
TagsFLAG tagExpressionInsectPromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
TagsmCherryExpressionInsectPromoternanosAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::FRT,attB}
Plasmid#182180Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertmTurquoise
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-EGFP::GW,attB}
Plasmid#182170Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertEGFP
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::GW,attB}
Plasmid#182169Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertmTurquoise
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3DsR
Plasmid#182204Purpose1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-DsRed
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G
Plasmid#146359PurposeInsect Expression of DmDCP2-PBDepositorInsertDmDCP2-PB (DCP2 Fly)
ExpressionInsectMutationContains an in-frame insertion that adds a Q afte…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2_E
Plasmid#146224PurposeInsect Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
ExpressionInsectMutationContains a K660E mutation compared to the sequenc…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F
Plasmid#146286PurposeInsect Expression of HsAGO2-F2V2DepositorInsertHsAGO2-F2V2 (AGO2 Human)
ExpressionInsectMutationContains F470V, F505V, K660E mutations compared t…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz-E958Q_AH
Plasmid#148884PurposeInsect Expression of DmHelz-E958QDepositorInsertDmHelz-E958Q (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA
Plasmid#175445PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTagsBiP Secretion Peptide and Thrombin-Protein AExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only