78,292 results

1. pLV-mScarlet-I

   Plasmid

   #159172

   Purpose

   To express a bright monomeric red FP for labelling and detection of mammalian cells

   Depositor

   Pantelis Tsoulfas

   Insert

   mScarlet-I
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Mutation

   Codons were optimized to Human codon usage

   Promoter

   CMV

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BRD2

   Plasmid

   #65376

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD2 (BRD2 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcBac1-Mb-sfGFP 150TAG

   Plasmid

   #197570

   Purpose

   Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   Pyl-tRNA (4 copies)
   

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA GFP10-ZIP-GFP11

   Plasmid

   #182243

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP-11
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-TO-dCas9

   Plasmid

   #75381

   Purpose

   dCas9

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   Sp dCas9
   

   Use

   Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMYH9-WT-V5

   Plasmid

   #183512

   Purpose

   Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Insert

   MYH9 (MYH9 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Inducible Caspex expression

   Plasmid

   #97421

   Purpose

   For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

   Depositor

   Steven Carr, Samuel Myers

   Article

   Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

   Inserts

   Caspex
   T2A-Gfp
   

   Use

   CRISPR

   Tags

   3xFLAG and V5

   Expression

   Mammalian

   Mutation

   dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF6-Acly(S455A)

   Plasmid

   #70767

   Purpose

   Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455A

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. VinculinTS

    Plasmid

    #26019

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin tension sensor
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCVhygro-F-Ezh2

    Plasmid

    #24926

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-hSV1_WT

    Plasmid

    #129483

    Purpose

    Mammalian expression of full-length human supervillin, isoform 1.

    Depositor

    Elizabeth Luna

    Article

    Smith et al Cytoskeleton (Hoboken). 2010 Jun;67(6):3

    Insert

    Supervillin (SVIL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TrkB mEGFP

    Plasmid

    #83952

    Purpose

    FRET donor for dynamically measuring TrkB activity in neurons

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Tropomyosin related kinase B (Ntrk2 Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Promoter

    CMV and U6/H1

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-TMEM106b-mApple

    Plasmid

    #179385

    Purpose

    Lentiviral vector for expression of TMEM106b fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    TMEM106b - mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid

    #212022

    Purpose

    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GianCreg (GOLGB1 Human)
    

    Tags

    td5StayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1099: Dual-sgRNA.Design 4

    Plasmid

    #159537

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with two guide RNA cassettes
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-ER alpha

    Plasmid

    #28230

    Purpose

    Mammalian expression of Estrogen Receptor alpha fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

    Insert

    Estrogen Receptor alpha (ESR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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