74,970 results

1. pAAV/D374Y-hPCSK9

   Plasmid

   #58379

   Purpose

   Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Insert

   proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D374Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-N-Solo-mStayGold-TJP1

   Plasmid

   #227300

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-TJP1

   Plasmid

   #227299

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. P1-EYFP-pA (Construct 5)

   Plasmid

   #55197

   Purpose

   Synthetic promoter responsive to gRNA1

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   EYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Ade MLP

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI-neo-mOVA

   Plasmid

   #25099

   Depositor

   Maria Castro

   Article

   Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

   Insert

   membrane bound ovalbumin
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBMN(CMV-copGFP-Puro-SMAR)

   Plasmid

   #80391

   Purpose

   expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Puromycine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLXRN-EGFP

   Plasmid

   #99207

   Purpose

   EGFP expressing bicistronic retroviral vector

   Depositor

   Peter Pedersen

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A Kozak sequence was incorporated to enhance tran…

   Promoter

   LTR

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-tdTomato (codon diversified)

   Plasmid

   #59462

   Purpose

   AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   codon diversified

   Promoter

   CAG

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-R-iLACCO1.1

   Plasmid

   #208026

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge