78,025 results

1. PX551

   Plasmid

   #60957

   Purpose

   pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162378

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE-Fast-FT

   Plasmid

   #31913

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. SV40-eGFP-Z1

   Plasmid

   #127638

   Purpose

   Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

   Depositor

   Jordan Green

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 shRUNX1 puro

   Plasmid

   #45816

   Depositor

   Kevin Janes

   Article

   Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

   Insert

   Runx1 (RUNX1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

   Plasmid

   #209728

   Purpose

   Expresses cytosolic high affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LeGO-G2

   Plasmid

   #25917

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroX-PTuner DD-linker-M.EcoGII

   Plasmid

   #122082

   Purpose

   Express M.EcoGII with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of M.EcoGII

   Expression

   Bacterial and Mammalian

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-TGNP-N-10

    Plasmid

    #54279

    Purpose

    Localization: Golgi Complex, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcdna nesprin TS

    Plasmid

    #68127

    Purpose

    nesprin tension sensor

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    mouse nesprin mini 2G (Syne2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS1982

    Plasmid

    #49113

    Purpose

    AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

    Insert

    ssAAV-Ple261-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CLDN5

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. MCP/PCP RNA Lantern

    Plasmid

    #226095

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-TO-dCas9-GFP

    Plasmid

    #64106

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. GRM1-DuET

    Plasmid

    #213300

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM1 (GRM1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/ZMYM2-FGFR1

    Plasmid

    #167486

    Purpose

    Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

    Depositor

    Nicholas Cross

    Article

    Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

    Insert

    ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
    

    Expression

    Mammalian

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-tdTomato

    Plasmid

    #99116

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-tdTomato
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Parkin-IRES-zeo

    Plasmid

    #61727

    Purpose

    Expression of human Parkin fused to mCherry

    Depositor

    Douglas Green, Stephen Tait

    Article

    Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

    Insert

    Parkin (PRKN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. SF3B3-1

    Plasmid

    #156004

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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