78,036 results

1. pLV-UbC-VP64-dCas9-VP64

   Plasmid

   #232179

   Purpose

   Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   VP64-dCas9-VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Sos1

   Plasmid

   #32920

   Depositor

   Dafna Bar-Sagi

   Article

   Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

   Insert

   SOS1 (SOS1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. SypHer mt

   Plasmid

   #48251

   Purpose

   pH sensor, targeted to mitochondria

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Tags

   2 mitochondrial targeting sequences

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 Lifeact-EGFP-2XNLS

   Plasmid

   #58467

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Lifeact
   2X NLS with 2X stop codons
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Perox-B

   Plasmid

   #209867

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA WDR24 pRK5

   Plasmid

   #46335

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   WDR24 (WDR24 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-CAG-gate-FLAG-IRES-GFP

   Plasmid

   #107398

   Purpose

   Lentiviral gateway destination vector

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGH044_mCherry

   Plasmid

   #85412

   Purpose

   lentiviral expression vector for mCherry

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-CMV-mouse Gfi1-GFP

   Plasmid

   #91892

   Purpose

   3rd generation lentiviral expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   Unknown

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hNox4

    Plasmid

    #69352

    Purpose

    expresses human Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    hNOX4 (NOX4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFFV_mNG3K(1-10)

    Plasmid

    #157993

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G27D, D42E, M128I, K153M, T170I

    Promoter

    SFFV

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available Since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Paxillin-22

    Plasmid

    #55114

    Purpose

    Localization: Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

    Insert

    Paxillin
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2MLS-HyperNova/pcDNA3

    Plasmid

    #221457

    Purpose

    Expressing HyperNova in mitochondria

    Depositor

    Kiwamu Takemoto

    Article

    Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

    Insert

    HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
    

    Expression

    Mammalian

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.3 N-MYC TIP60

    Plasmid

    #213003

    Purpose

    expresses human TIP60

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    KAT5 (KAT5 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF.myc.ER-E2-Crimson

    Plasmid

    #38770

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Tags

    ER retention signal (KDEL), ER signal sequence (M…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. SP1-SB100X

    Plasmid

    #154887

    Purpose

    Sleeping Beauty transposase fused to SP1

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    SP1-Sleeping Beauty
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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