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pcDNA3-GFP-MT2A

Plasmid

#11613

Depositor

Steven Johnson

Insert

metallothionein 2A (MT2A Human)

Tags

GFP

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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gag-TadCBEd

Plasmid

#225144

Purpose

Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Beta1-N-18

Plasmid

#55064

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-YY1

Plasmid

#169018

Purpose

Expression of YY1 driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human YY1 (YY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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Lenti-PEAR-mCherry

Plasmid

#228892

Purpose

All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Insert

EGFP-IRES-mScarlet

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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human MMP-2 promoter

Plasmid

#53432

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

Insert

MMP2 promoter (MMP2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP2

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpot-Tag-GFP-GPI

Plasmid

#117061

Purpose

For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag

Depositor

Felix Hartlepp

Insert

GPI-anchored GFP

Tags

GPI-anchor and Spot-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RUNX1

Plasmid

#192928

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX1 (RUNX1 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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ActTS-GR

Plasmid

#79774

Purpose

FRET-based actinin tension sensors

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

ActTS-GR

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLIX403-hNOS2

Plasmid

#110800

Purpose

tet-inducible NOS2 expression

Depositor

Edward Morgan

Article

Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

Insert

NOS2 (NOS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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ER-B

Plasmid

#209870

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10B

Plasmid

#35921

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC7A11

Plasmid

#132244

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-FLAG-Ku70

Plasmid

#46957

Purpose

Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV- κLight1.3

Plasmid

#201223

Purpose

Express kLight1.3 biosensor in mammalian cells

Depositor

Lin Tian

Article

Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

Insert

κLight1.3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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