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pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

Plasmid

#100852

Purpose

AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

Plasmid

#110057

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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TATA-GFP

Plasmid

#110833

Purpose

negative control

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40

Plasmid

#110154

Purpose

Expression of human BHLHE40 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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mCarmine in pcDNA3

Plasmid

#109486

Purpose

mammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP.90 beta

Plasmid

#108221

Purpose

Mammalian expression vector for EGFP fusion to human Hsp90 beta

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EGFP-Hsp90b (HSP90AB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6s

Plasmid

#105714

Purpose

Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

Depositor

Rylan Larsen

Has Service

AAV8

Insert

GCaMP6s

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

Ef1a, Human elongation factor-1 alpha

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-ABE(7.10)

Plasmid

#108387

Purpose

Mammalian expression vector for xCas9(3.6)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-NCre

Plasmid

#106368

Purpose

N-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

NCre

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-CyOFP1

Plasmid

#105799

Purpose

Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-CyOFP1

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP7c variant 1513-WPRE

Plasmid

#105321

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV1

Insert

jGCaMP7c variant 1513

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pC0045-EF1a-PguCas13b-NES-HIV

Plasmid

#103861

Purpose

Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PguCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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LAMP-mCherry-CRY2

Plasmid

#102249

Purpose

Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGF1-4eCOL2A1

Plasmid

#97210

Purpose

Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

Depositor

Ryan Porter

Article

De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

Insert

4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

COL2A1 regulatory elements

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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CMV Marina-T2A-nls-mCherry

Plasmid

#74216

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

fluorescent protein voltage sensor

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK CA Rac1

Plasmid

#84605

Purpose

Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only