78,093 results

1. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2_Firefly_degron-60A

   Plasmid

   #200396

   Purpose

   A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Firefly luciferase - degron
   

   Expression

   Mammalian

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIR-FLAG-MLL-AF9

   Plasmid

   #71444

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-AF9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EMTB-3XGFP

   Plasmid

   #26741

   Depositor

   William Bement

   Article

   Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Probe for microtubules

   Tags

   3x EGFP and Myc

   Expression

   Mammalian

   Mutation

   Only contains the microtubule binding-domain of e…

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERP2-EGR1

   Plasmid

   #192913

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EGR1 (EGR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-Luc2-P2A-copGFP

   Plasmid

   #72485

   Purpose

   Express Luc2 and copGFP

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-copGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. YFP-GID1

   Plasmid

   #37305

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   See comments
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-IQGAP1

   Plasmid

   #30112

   Depositor

   David Sacks

   Article

   Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Tags

   tandem EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCS-3_Endofin_LgBiT

   Plasmid

   #182574

   Purpose

   This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

   Depositor

   Bradley McConnell

   Article

   Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

   Insert

   zinc finger FYVE-type (ZFYVE16 Human)
   

   Tags

   LgBiT (Large BiT)

   Expression

   Mammalian

   Mutation

   FYVE domain Q739-K806

   Promoter

   Herpes simplex virus (HSV)

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid

    #175181

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-KRAB-dCas9-IRES-GFP

    Plasmid

    #85556

    Purpose

    2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. RfxCas13d-backbone

    Plasmid

    #165078

    Purpose

    Expression of RfxCas13d sgRNAs from a U6 promoter

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    Scaffold gRNA
    

    Expression

    Mammalian

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-PIK3R1

    Plasmid

    #174191

    Purpose

    Expresses PIK3R1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154786

    Purpose

    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Fly, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and mCherry

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTagBFP2-Lysosomes-20

    Plasmid

    #55308

    Purpose

    Localization: Lysosome Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    LAMP1
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PB-NES-ZapCV2-Zinc dead

    Plasmid

    #203850

    Purpose

    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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