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82,530 results

Showing: 7201 - 7220 of 82530 results

  2. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168122-plasmid-map-sequence-id-332862

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  7. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161927
    Purpose
    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and Citrine repression reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161927-plasmid-map-sequence-id-324349

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  10. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Publication
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158230-plasmid-map-sequence-id-317503

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  16. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    157854-plasmid-map-sequence-id-307627

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Showing: 7201 - 7220 of 82530 results