76,036 results

1. pBS-EF1afull-H2B-mCherry-P2A-Fluc

   Plasmid

   #207253

   Purpose

   Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   H2B-mCherry-P2A-Fluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha_full

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lck-GFP

   Plasmid

   #61099

   Purpose

   Expression of a membrane-targeted form of eGFP

   Depositor

   Steven Green

   Article

   Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

   Insert

   Lck-GFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBB HA RelA

   Plasmid

   #74892

   Purpose

   Express RelA in mammalian cells

   Depositor

   Ezra Burstein, Colin Duckett

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   RelA (RELA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

   Plasmid

   #205161

   Purpose

   express β-ACTIN-3′UTR-24xMS2

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   β-ACTIN-3′UTR-24xMS2
   

   Use

   Tags

   tagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-GFP-P2A-MCS (Neo)

   Plasmid

   #89181

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nsp12-EGFP

   Plasmid

   #165116

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp12 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HIF1 alpha

   Plasmid

   #21101

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-DDR1-V5/HIS

   Plasmid

   #201100

   Purpose

   expression of human DDR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

    Plasmid

    #149722

    Purpose

    Lentiviral expression of crRNA with GFP and a neomycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Puro.Cre empty vector

    Plasmid

    #17408

    Purpose

    Lentiviral expression of Cre from the mouse PGK promoter

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Calreticulin

    Plasmid

    #191940

    Purpose

    Expression of calreticulin

    Depositor

    Luca Scorrano

    Insert

    Calreticulin (Calr Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAdEasy-2

    Plasmid

    #16401

    Purpose

    Adenoviral vector for use in AdEasy System, for use with large inserts.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209726

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVTNT PINK1 N-myc

    Plasmid

    #13313

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-IRES-Hygro_RGECO1.2

    Plasmid

    #164592

    Purpose

    Stable integration and expression of RGECO1.2 in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    R-GECO1.2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hROSA26 CRISPR-pX330

    Plasmid

    #105927

    Purpose

    hROSA26 targeting CRISPR plasmid

    Depositor

    Masato Kanemaki

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    hROSA26 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKRaION2-N1

    Plasmid

    #177802

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCKO

    Plasmid

    #73311

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge