75,966 results

1. EGFP-OSBP-PH

   Plasmid

   #49571

   Purpose

   expresses the plekstrin homology domain of OSBP in mammalian cells

   Depositor

   Marci Scidmore

   Article

   Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

   Insert

   OSBP-PH (AA 91-179) (OSBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-FFSS-Cyclin D1 (T286A)

   Plasmid

   #215152

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   With T286A mutation.

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kras-Src FRET biosensor

   Plasmid

   #78302

   Purpose

   FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

   Insert

   Src biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF3056

   Plasmid

   #144532

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   THRB (THRB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ALK6 Q203D

   Plasmid

   #80883

   Purpose

   mammalian expression of ALK6 Q203D

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK6 (Bmpr1b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q203D (constitutively active)

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pLSSmOrange-N1

   Plasmid

   #37130

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SHP2 CS

   Plasmid

   #8382

   Depositor

   Ben Neel

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C459S, mutation of essential catalytic cysteine

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-Flag HBx

   Plasmid

   #42596

   Depositor

   Ding Xue

   Article

   Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

   Insert

   Hepatitis B virus protein X
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. evoBE4-NG

   Plasmid

   #226852

   Purpose

   Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   evoBE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCMV_DENV3_PrME

    Plasmid

    #207265

    Purpose

    Mammalian expression of Dengue virus 3 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV3_PrME (POLY Dengue virus 3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3xFLAG CMV14 / BRAF-V600E

    Plasmid

    #131723

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    CMV

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-GGA1

    Plasmid

    #178459

    Purpose

    Expresses GGA1 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

    Insert

    GGA1 (GGA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. iRFP682-Smad2

    Plasmid

    #118943

    Purpose

    Mammalian expression of human Smad2 protein

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    iRFP682-Smad2 (SMAD2 Human)
    

    Use

    Tags

    iRFP682

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MigR1-AE

    Plasmid

    #12431

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF2700

    Plasmid

    #142978

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVITRO1-SS-345

    Plasmid

    #68785

    Purpose

    R->A Sensor pair 1 with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V2 R-->A mutant
    N-Terminal Sensor 1 V2 R-->A mutant
    mCherry
    

    Use

    Tags

    NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-p53-shRNA-427

    Plasmid

    #25636

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-427 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge