75,820 results

1. pMMLV-EGFP-P2A-Puro

   Plasmid

   #160226

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   EGFP-P2A-PuroR
   

   Use

   Retroviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdp43-EGFP construct4

   Plasmid

   #28197

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa216-414 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVHA EED wt

   Plasmid

   #24231

   Purpose

   Mammalian expression of human EED with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EED (EED Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

   Plasmid

   #161926

   Purpose

   Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   rTetR(SE-G72P)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-EGFP-Tau AP

   Plasmid

   #46905

   Purpose

   expresses EGFP tagged Tau AP in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-FLAG-SBP-STAG2 (WT)

   Plasmid

   #73963

   Purpose

   STAG2 cDNA (CCDS43990) wild type

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. HDAC8 Flag

   Plasmid

   #13825

   Depositor

   Eric Verdin

   Article

   Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

   Insert

   HDAC8 (HDAC8 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-p62-Silent_Mutation_WT

   Plasmid

   #187986

   Purpose

   human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   p62 (NUP62 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutation for resistance to sip62 #5

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CCR2-DuET

   Plasmid

   #213198

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR2 (CCR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-HA-Nrxn1beta AS4(-)

    Plasmid

    #59409

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRY2olig-NONO

    Plasmid

    #218922

    Purpose

    Expression of CRY2olig-mCherry fused to NONO

    Depositor

    Tatyana Shelkovnikova

    Insert

    CRY2olig-mCherry tagged NONO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Use

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Kaede-N1

    Plasmid

    #54726

    Purpose

    Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Kaede

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

    Plasmid

    #134859

    Purpose

    Expresses EGFP-tagged ERGIC marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    ERGIC53 (Lman1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTagRFP-Membrane-1

    Plasmid

    #57992

    Purpose

    Localization: Plasma Membrane, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    N-terminal 20 Aas MLS from Neuromodulin and mTagR…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+) K-Ras(WT)

    Plasmid

    #224283

    Purpose

    Cell transfection and expression of K-Ras

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-mASS1

    Plasmid

    #34576

    Depositor

    Peter Murray

    Insert

    ASS1 (Ass1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRXIP-GFP-Spo20(PABD)

    Plasmid

    #221758

    Purpose

    Stable expression of GFP-Spo20(PABD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Spo20 (SOS1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem Spo20(PABD) (amino acids 422-551)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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