78,133 results

1. pcDNA3.1(+)-AKAP95-FRB

   Plasmid

   #138254

   Purpose

   Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   AKAP95-FRB (AKAP8 Human)
   

   Tags

   FRB rapamycin binding domain and HA epitope

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-DN-hCUL4A-FLAG

   Plasmid

   #15821

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin4A (CUL4A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N440

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57-MCS1-P2A-MCS2 (RFP)

   Plasmid

   #80923

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. iCAP-BI-28-KanR

   Plasmid

   #203532

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI28 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-HA-rat Nrxn1alpha

   Plasmid

   #58266

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1alpha

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1alpha (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-3HA-Mefv iso1

   Plasmid

   #92105

   Purpose

   Expression in eukaryotic cells

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   Mefv isoform1 (Mefv Mouse)
   

   Tags

   3xHA

   Expression

   Mammalian

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-TK-5UTR-BsmBI-Luciferase

   Plasmid

   #114670

   Purpose

   For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

   Depositor

   Lynne Postovit

   Article

   Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

   Insert

   LacZ
   

   Use

   Luciferase

   Tags

   for cloning 5' UTR-Luciferase fusions

   Expression

   Mammalian

   Promoter

   lac promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCiPS

   Plasmid

   #60011

   Purpose

   Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

   Depositor

   David Russell

   Article

   Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

   Insert

   Pol
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT-TODestGFP_RNAseH1

    Plasmid

    #65784

    Purpose

    expresses GFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Tags

    GFP and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Venus1-K49R-STAT3

    Plasmid

    #123166

    Purpose

    Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    K49R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Venus2-K49R-STAT3

    Plasmid

    #123167

    Purpose

    Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    K49R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus1-K140R-STAT3

    Plasmid

    #123168

    Purpose

    Expresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    K140R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Venus2-K140R-STAT3

    Plasmid

    #123169

    Purpose

    Expresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    K140R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus2-K685R-STAT3

    Plasmid

    #123171

    Purpose

    Expresses K695R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    K685R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG mitoYFP

    Plasmid

    #168508

    Purpose

    Expression of mito-EYFP (mitochondrial matrix targeted EYFP) for visualization of mitochondrial dynamics

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    mitoYFP
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-N1-cofilin

    Plasmid

    #186750

    Purpose

    Expresses wildtype mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-Cyclin D1

    Plasmid

    #215050

    Purpose

    Expresses EGFP-Cyclin D1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid

    #212196

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

    Depositor

    Stacey Edwards

    Article

    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

    Insert

    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    

    Use

    CRISPR and Lentiviral

    Tags

    HA, NLS, and blasticidin S deaminase

    Expression

    Mammalian

    Promoter

    EF-1α promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-Zeo

    Plasmid

    #75088

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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