78,133 results

1. pT2/SV-Venus-SIINFEKL

   Plasmid

   #111624

   Purpose

   Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

   Depositor

   Jon Yewdell

   Insert

   Venus-LEQLE-SIINFEKL-TEW
   

   Tags

   LEQLE-SIINFEKL-TEW

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-Blue FP

   Plasmid

   #52115

   Purpose

   a bicistronic IRES-Blue FP containing retroviral vector

   Depositor

   Dario Vignali

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-Blue FP

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 TaGu N-1F

   Plasmid

   #203762

   Purpose

   For expression of N-flag tagged TaGu R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti guide Thy1.1

   Plasmid

   #128063

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCL1177

   Plasmid

   #217961

   Purpose

   Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Histone H3 (HIST2H3PS2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-SPNS1_STOP

   Plasmid

   #161439

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SPNS1 (SPNS1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pRubiG-T2A-Cas9

   Plasmid

   #75348

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. DV-GFP-Rab38-WT-pDEST53

   Plasmid

   #15669

   Depositor

   William Pavan

   Article

   Loftus et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   Rab38 (Rab38 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   wildtype Rab38 sequence

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. EIF2AK2 gRNA (BRDN0001146342)

   Plasmid

   #75637

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP-Rac1

    Plasmid

    #11391

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-mStayGold-CANX

    Plasmid

    #227280

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p189

    Plasmid

    #65207

    Purpose

    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Expression

    Bacterial and Mammalian

    Mutation

    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lentiviral-FOP-dGFP-reporter

    Plasmid

    #14885

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    mutant LEF-1/TCF-responsive promoter (Ctnnb1 )
    

    Use

    Lentiviral

    Tags

    d2 eGFP

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSF107mT-GATEWAY-3'-FLAG

    Plasmid

    #67619

    Purpose

    A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Flag

    Expression

    Mammalian

    Promoter

    CMV and SP6

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171385

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-RESCUE-S
    

    Expression

    Mammalian

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL Neo Myr Flag BTK

    Plasmid

    #20432

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    BTK (BTK Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pHL-avitag3

    Plasmid

    #99847

    Purpose

    mammalian expression with secretive signal and avi tag for biotinylation

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Tags

    Avi

    Expression

    Mammalian

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HRE-oUnaG

    Plasmid

    #201712

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Expression

    Mammalian

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-RhoA-wt_mScarlet-i_SGFP2

    Plasmid

    #85071

    Purpose

    Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
    

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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