78,133 results

1. pEF1a_CD WT TET1

   Plasmid

   #124082

   Purpose

   Expression of wild-type catalytic domain only form of TET1

   Depositor

   Silvia Monticelli

   Article

   Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

   Insert

   TET1 catalytic domain (TET1 Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   EF1 alpha

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-NGN2-EMX1 BSD-mApple

   Plasmid

   #182311

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Rab21

   Plasmid

   #83421

   Purpose

   Mouse Rab21 (wild type)

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACS129

   Plasmid

   #204594

   Purpose

   Histone H3.1-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.1 (H3C3 Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.1-V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIMTOR A757

   Plasmid

   #140829

   Purpose

   AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR A757 (ULK1 Human)
   

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GeNL/pcDNA3

   Plasmid

   #85200

   Purpose

   Green color variant of bright luminescent protein

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   Green enhanced Nano-lantern
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-AR

   Plasmid

   #111215

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Insert

   AR (Ar Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDx_mScarlet3-SYFP2

   Plasmid

   #189764

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

    Plasmid

    #176541

    Purpose

    human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB omegaRNA scaffold
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi 41BBL CD86 HLA-A201

    Plasmid

    #183248

    Purpose

    Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Inserts

    CD86, 41BBL
    HLA-A2 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3xFLAG CMV14 / BRAF

    Plasmid

    #131710

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPCX-PECAMTL

    Plasmid

    #45851

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    PECAM1 (PECAM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA 3 5' cMyc SMC1 wt

    Plasmid

    #32363

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCardinal-C1

    Plasmid

    #54799

    Purpose

    Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mCardinal

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pHCMV-SS-antiMoonTagNb-TM

    Plasmid

    #198305

    Purpose

    Displays anti-MoonTag nanobody from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiMoonTagNb-TM
    

    Expression

    Mammalian

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD649-HAsp-SIGLEC15-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156698

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC15 (SIGLEC15 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-HA-AR-DBD-LBD

    Plasmid

    #171237

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP VAMP7 (102-220)

    Plasmid

    #42314

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ1-102

    Promoter

    CMV

    Available Since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only