80,246 results

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AAV-Camk2-NES-Twitch-GR

Plasmid

#173044

Purpose

TnC-based calcium sensor with green-red FRET pair ratiometric readout

Depositor

Yiyang Gong

Article

Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

Has Service

AAV2

Insert

NES-Twitch-GR

Use

AAV

Expression

Mammalian

Promoter

CamK2

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag3B-CCre

Plasmid

#106369

Purpose

C-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a-MICB*005-IRES-ZsGreen

Plasmid

#114008

Purpose

Induces expression of human MICB allele 005 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICB*005 (MICB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3-mRuby3

Plasmid

#203492

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC4A10_STOP

Plasmid

#161474

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A10 (SLC4A10 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA Myc DBC1

Plasmid

#35096

Depositor

Osamu Hiraike

Article

Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

Insert

deleted in breast cancer 1 (CCAR2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mScarlet-LMNB1

Plasmid

#207771

Purpose

Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

Plasmid

#186699

Purpose

Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD86-ST3-His

Plasmid

#210664

Purpose

Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CCND1

Plasmid

#172632

Purpose

Expresses untagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-UbC-Blast-2A-STING-mNeonGreen

Plasmid

#227185

Purpose

Lentiviral expression plasmid encoding STING-mNeonGreen under UbC promoter

Depositor

Nir Hacohen

Article

Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

UbC

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

Plasmid

#187459

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Article

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D1-25-msfGFP

Plasmid

#180333

Purpose

mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D1-25

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2 Spike

Plasmid

#185604

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

Insert

SARS-CoV-2 BA.2 Spike (S )

Expression

Mammalian

Mutation

T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

July 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV3TAG8li-hRNF160-3FLAG

Plasmid

#159138

Purpose

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

listerin E3 ubiquitin protein ligase 1 (LTN1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-a1AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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