79,341 results

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pRK5-HA GST RagD wt

Plasmid

#19307

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pIS1_Opt_Renilla

Plasmid

#210571

Purpose

Expresses Optimal Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla

Use

Luciferase

Expression

Mammalian

Promoter

HSV TK; T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-N GFP

Plasmid

#37855

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

GFP

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-AktAR2

Plasmid

#64932

Purpose

Enhanced FRET-based biosensor for monitoring Akt activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

AktAR2

Tags

Cerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-YTHDC2

Plasmid

#222558

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

YTHDC2 (YTHDC2 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-NSP2SA11

Plasmid

#89169

Purpose

Express Rotavirus SA11 NSP2 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP2

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKH3

Plasmid

#12555

Depositor

Ian Macara

Article

Mattingly et al Nature. 1996 Jul 18. 382(6588):268-72.

Type

Empty backbone

Tags

3xHA

Expression

Mammalian

Available Since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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2C-EGFP-t2a-mCD4

Plasmid

#216213

Purpose

Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

Depositor

Michelle Percharde

Article

Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

Insert

CD4 (Cd4 Mouse)

Tags

GFP-T2A

Expression

Mammalian

Mutation

CD4 transmembrane domain only

Promoter

MERVL

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

Plasmid

#215010

Purpose

Express a Mitophagy reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

pSu9 (Presequence of Fo-ATPase subunit 9)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pmVen-RecQL4

Plasmid

#211401

Purpose

mammalian expression of human RecQL4 N-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

RecQL4 (RECQL4 Human)

Tags

mVenus

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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p52sheLL

Plasmid

#46950

Purpose

HPV52 L1 and L2 Bicistronic, too large to self-package

Depositor

John Schiller

Article

Kondo et al J Med Virol. 2008 May;80(5):841-6. doi:

Insert

HPV52L1+L2 (L1, L2 Human)

Expression

Mammalian

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pRS3 (CK2alpha, CK2beta)

Plasmid

#27092

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Promoter

bidirectional tet-responsive promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP37

Plasmid

#22602

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP37 (USP37 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

Plasmid

#199359

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-PcKRS

Plasmid

#140022

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

PcKRS (pylS Methanosarcina bakeri)

Tags

FLAG

Expression

Mammalian

Mutation

M241F, A267S, Y271C, L274M

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302 EGFP-V5

Plasmid

#141348

Purpose

Expresses V5 tagged GFP

Depositor

Kevin Janes

Insert

EGFP

Use

Lentiviral

Tags

V5 tag

Expression

Mammalian

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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