78,292 results

1. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-tmpknot-GG-acceptor

   Plasmid

   #174039

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHiUGE TurboID-HA donor (all-in-one) ORF0

   Plasmid

   #200383

   Purpose

   Tagging endogenously expressed proteins with TurboID-HA, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREP-8xARE-GFP-SV40-BFP

   Plasmid

   #134910

   Purpose

   NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Inserts

   8xARE- minimal promoter - GFP
   TagBFP
   

   Expression

   Mammalian

   Promoter

   8xARE - minimal promoter and SV40

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mCardinal

   Plasmid

   #51311

   Purpose

   Expresses far-red fluorescent protein mCardinal in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mCardinal
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRFP-Lact-C2

   Plasmid

   #74061

   Purpose

   Fluorescent biosensor for phosphatidylserine.

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 (MFGE8 Bovine)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

   Plasmid

   #196703

   Purpose

   Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 D210N
   

   Expression

   Mammalian

   Mutation

   D210N, catalytically inactive

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1759

    Plasmid

    #143845

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ALYREF (ALYREF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFLAG_WT MDA5

    Plasmid

    #216798

    Purpose

    Expresses wild type (wt) melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MCS-BioID2-HA

    Plasmid

    #74224

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1 Lifeact-EGFP

    Plasmid

    #58470

    Purpose

    Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Lifeact
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234446

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBbi-RB

    Plasmid

    #60522

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. ACBE-Q

    Plasmid

    #204608

    Purpose

    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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