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pMIG-w

Plasmid

#12282

Purpose

a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus

Depositor

Luk Parijs

Article

Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

Type

Empty backbone

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 28, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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AIO-mCherry

Plasmid

#74120

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pALB-GFP

Plasmid

#55759

Purpose

Fluorescent marker for hepatocytic lineage

Depositor

Snorri Thorgeirsson

Article

Heo et al Hepatology. 2006 Dec;44(6):1478-86.

Insert

albumin enhancer/promoter (Alb Mouse)

Expression

Mammalian

Promoter

Mouse Albumin Enhancer/Promoter

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-tdT

Plasmid

#59171

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Tags

6xHis and CAAX

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a

Plasmid

#25030

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP1a (DCP1A Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 23, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Twist ER

Plasmid

#18799

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

twist (Twist1 Mouse)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

V5

Expression

Mammalian

Mutation

P101S

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMIG-hKLF4

Plasmid

#17227

Depositor

George Daley

Article

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

Kruppel-like factor 4 (gut) (KLF4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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IKK-2 K44M

Plasmid

#11104

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

K44M, kinase inactive

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV flag ERR alpha

Plasmid

#10975

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

Insert

ERR alpha (ESRRA Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-SHP2 WT

Plasmid

#8381

Depositor

Ben Neel

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

Plasmid

#71396

Purpose

2Gi2R assay

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Inserts

2xGFP:NES
2xRFP:NLS

Use

Lentiviral

Tags

IRES

Expression

Mammalian

Promoter

EF1alpha and IRES

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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