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LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

Plasmid

#192412

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

Plasmid

#192413

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB230

Plasmid

#68621

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

tdTomato

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBCC155

Plasmid

#74995

Purpose

CFP-based BiFC vector for transient expression of CC (155-238) in plants

Depositor

Masamitsu Wada, Yutaka Kodama

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (155–239)/CC155

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBCN155

Plasmid

#74994

Purpose

CFP-based BiFC vector for transient expression of CN (1-155) in plants

Depositor

Masamitsu Wada, Yutaka Kodama

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (1–155)/CN155

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCO487

Plasmid

#139730

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, H3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_B_AtUbi10:NnH3H:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL12015

Plasmid

#117506

Purpose

AtUBI10 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pUBI10:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

AtUBI10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2+pCmYLCV911

Plasmid

#170878

Purpose

Phytobrick - pCmYLCV911

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pCmYLCV911

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+pG10-90

Plasmid

#170879

Purpose

Phytobrick - pG10-90

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pG10-90

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [M1_n-1] (GB1210)

Plasmid

#75411

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [M1_n-1]

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJIT163-eAFID-3

Plasmid

#167360

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3Bctd-Cas9-UDG-T2A-AP lyase

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM858a/b

Plasmid

#84177

Purpose

target miRNA858 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM858a/b

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc

Plasmid

#86158

Purpose

FLS2 expression

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

3xmyc

Expression

Plant

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB126

Plasmid

#68580

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

No Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N

Plasmid

#189649

Purpose

rbc L 294 Left_G1397 N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Use

Tags

HA, lifeact, and p2a

Expression

Plant

Mutation

Promoter

35S

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ269C2

Plasmid

#164716

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible haid-cbe_v04 entry clo…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pNapin-eGFP

Plasmid

#127797

Purpose

Napin Promoter driven eGFP expression

Depositor

Scott Poethig

Article

Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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MIGS5.1 (FF572)

Plasmid

#35253

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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lvl0 nluc CDS

Plasmid

#153404

Purpose

Nanoluciferase coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

nanoluciferase CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha2

Plasmid

#68225

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega1

Plasmid

#68234

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB1_omega2

Plasmid

#68235

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB123

Plasmid

#68577

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM156/157

Plasmid

#84083

Purpose

target miRNA156/157 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM156/157

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_2

Plasmid

#91160

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pNC-Cam2304-RNAi

Plasmid

#193400

Purpose

Nimble cloning-compatible vector for plant RNAi

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-KSE401

Plasmid

#193435

Purpose

Nimble cloning-compatible vector for genome editing in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-BiFC-VD2

Plasmid

#193419

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge