4,457 results

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB432

Plasmid

#68697

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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ARF17 pGreenII0129

Plasmid

#12080

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

ARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pGSPE-ccdb

Plasmid

#242424

Purpose

for pegRNA cloning

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

ccdb

Use

CRISPR

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGGA-FT guide RNA1_7

Plasmid

#246797

Purpose

A GreenGate entry vector containing a guide RNA expression cassette targeting the AtFT gene with 7 different gRNAs

Depositor

Danhua Jiang

Insert

AtU6-1 promoter-FT guide RNA1-AtU6-1 promoter-FT guide RNA3- (FT Synthetic, Mustard Weed)

Use

CRISPR; Greengate cloning entry vector

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pART27

Plasmid

#247986

Purpose

Binary vector to receive a NotI fragment from pART7 with a gene to be stably expressed in plants via agrobacterium-mediated transformation.

Depositor

Andrew Gleave, Christoph Plieth

Article

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-SRDX (M30)

Plasmid

#239484

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-SRDX

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-BFP (M39)

Plasmid

#239485

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-cSRDX (M40)

Plasmid

#239486

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-SRDX

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-p300 (D18)

Plasmid

#239489

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-p300

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1cd (D24)

Plasmid

#239490

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1cd

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU6-HV5

Plasmid

#239470

Purpose

Encodes a gRNA expression cassette driven by the AtU6 promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U6-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU3b-HV5

Plasmid

#239477

Purpose

Encodes a gRNA expression cassette driven by the AtU3b promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U3b-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-At7SL-HV5

Plasmid

#239478

Purpose

Encodes a gRNA expression cassette driven by the At7SL promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

7SL-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p1300-dCas9-HV5

Plasmid

#239479

Purpose

An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

UBQ-dCAS9

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-KYP (M6)

Plasmid

#239480

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-dCas9-2XNLS-XTEN16-BFP-KYP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9 (M14)

Plasmid

#239482

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-KRAB (M22)

Plasmid

#239483

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-KRAB

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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ttLbCas12a-intron

Plasmid

#241901

Purpose

ttLbCas12a (LbCas12a-D156R)-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

ttLbCas12a (LbCas12a-D156R)-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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LbCas12a-RRV-intron

Plasmid

#241902

Purpose

LbCas12a-RRV-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

LbCas12a-RRV-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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LbCas12a-intron

Plasmid

#241900

Purpose

LbCas12a-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

LbCas12a-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC0_Empty

Plasmid

#243799

Purpose

pAVEC0 backbone with no fluorophores or tags

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Agrobacterium mediated transformation

Expression

Plant

Promoter

35S

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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nYFP-pBSDONR_L1-L4

Plasmid

#247584

Purpose

Gateway donor vector carrying an N-terminal YFP tag, flanked by attL1 and attL4 sites for MultiSite Gateway recombination.

Depositor

Juan Dong

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Aug 12;12

Insert

nYFP

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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cYFP-pBSDONR_L1-L4

Plasmid

#247585

Purpose

Gateway donor vector carrying a C-terminal YFP tag, flanked by attL1 and attL4 sites for MultiSite Gateway recombination

Depositor

Juan Dong

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Aug 12;12

Insert

cYFP

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-Ruby

Plasmid

#234918

Purpose

This all-in-one vector is used to express the Ruby gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Ruby

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-GUS

Plasmid

#234916

Purpose

This all-in-one vector is used to express the GUS gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

GUS

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-5

Plasmid

#246288

Purpose

Evaluation of AtU6.1m2 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.1m2 promoter

Use

CRISPR

Expression

Plant

Mutation

A-to-C (-63 from TSS) and G-to-T (-57) within USE

Promoter

AtU6.1m2 promoter

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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